• Record: found
  • Abstract: found
  • Article: not found

Long-term subculture of human keratinocytes under an anoxic condition.

Journal of bioscience and bioengineering

Tissue Engineering, methods, Cell Hypoxia, Cell Culture Techniques, physiology, Cell Proliferation, Cell Survival, Cells, Cultured, Humans, Keratinocytes, cytology, Oxygen, metabolism, Time Factors, Adaptation, Physiological

Read this article at

      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


      The serial subculturing of human keratinocyte cells under the anoxic and normoxic conditions was examined. The cumulative number of population doublings in the subcultures under the former condition increased 2.1-fold while maintaining an appreciable growth rate of cells, as compared with that under the latter condition. Moreover, the migration ability, which was estimated by the rotation rate of paired cells, was maintained accompanied by fully developed filopodia of F-actin filaments under the anoxic condition, despite of the poor development of stress fibers at the center of the cellular body. The cells passaged under the anoxic condition possessed the sufficient clonogenic potential to form epithelial sheets, supporting the view that the long-term subculture of keratinocytes under the anoxic condition can be applied for cell expansion in the practical production of epithelial sheets.

      Related collections

      Author and article information



      Comment on this article