In vitro and in vivo studies of an aqueous extract of Matricaria recutita (German chamomile) on the radiolabeling of blood constituents, on the morphology of red blood cells and on the biodistribution of the radiopharmaceutical sodium pertechnetate

Abstract

Background:

Natural products might alter the labeling of blood constituents with technetium-99m ( ^99mTc) and these results may be correlated with modifications of the shape of the red blood cells (RBC). The biodistribution of radiopharmaceuticals can be also altered.

Objective:

This investigation aimed to determine biological effects of an aqueous extract of chamomile (CE).

Materials and Methods:

To study the effect of the CE on the labeling of blood constituents with ^99mTc, in vitro and in vivo assays were performed. The effect of the CE on the morphology of RBC was observed under light microscope. The images were acquired, processed, and the perimeter/area ratio of the RBC determined. To analyze the effect of the CE on biodistribution of the sodium pertechnetate (Na ^99mTcO ₄) in Wistar rats, these animals were treated or not with a CE. Na ^99mTcO ₄ was injected, the rats were sacrificed, the organs were removed, weighted and percentage of radioactivity/gram calculated.

Result:

In the in vitro experiment, the radioactivity on blood cells compartment and on insoluble fractions of plasma was diminished. The shape and the perimeter/area ratio of the RBC were altered in in vitro assays. An increase of the percentage of radioactivity of Na ^99mTcO ₄ was observed in stomach after in vivo treatment.

Conclusion:

These results could be due to substances of the CE or by the products of the metabolism of this extract in the animal organism. These findings are examples of drug interaction with a radiopharmaceutical, which could lead to misdiagnosis in clinical practice with unexpected consequences.