• Record: found
  • Abstract: found
  • Article: not found

RGMa inhibits neurite outgrowth of neuronal progenitors from murine enteric nervous system via the neogenin receptor in vitro.

Journal of Neurochemistry

Antimetabolites, pharmacology, Bromodeoxyuridine, Cell Proliferation, Enteric Nervous System, cytology, growth & development, Female, GPI-Linked Proteins, HEK293 Cells, Humans, Immunohistochemistry, In Situ Hybridization, Intestines, innervation, Membrane Proteins, antagonists & inhibitors, physiology, Reverse Transcriptase Polymerase Chain Reaction, Mice, Mice, Inbred C57BL, Mice, Knockout, Nerve Tissue Proteins, Neural Stem Cells, drug effects, Neurites, Neuroglia, Neurons, Pregnancy, RNA, biosynthesis, genetics, Receptors, Cell Surface, metabolism, Animals

Read this article at

      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


      The enteric nervous system (ENS) in vertebrate embryos is formed by neural crest-derived cells. During development, these cells undergo extensive migration from the vagal and sacral regions to colonize the entire gut, where they differentiate into neurons and glial cells. Guidance molecules like netrins, semaphorins, slits, and ephrins are known to be involved in neuronal migration and axon guidance. In the CNS, the repulsive guidance molecule (RGMa) has been implicated in neuronal differentiation, migration, and apoptosis. Recently, we described the expression of the subtypes RGMa and RGMb and their receptor neogenin during murine gut development. In the present study, we investigated the influence of RGMa on neurosphere cultures derived from fetal ENS. In functional in vitro assays, RGMa strongly inhibited neurite outgrowth of differentiating progenitors via the receptor neogenin. The repulsive effect of RGMa on processes of differentiated enteric neural progenitors could be demonstrated by collapse assay. The influence of the RGM receptor on ENS was also analyzed in neogenin knockout mice. In the adult large intestine of mutants we observed disturbed ganglia formation in the myenteric plexus. Our data indicate that RGMa may be involved in differentiation processes of enteric neurons in the murine gut.

      Related collections

      Author and article information



      Comment on this article