Gravitropism is an important strategy for the adaptation of plants to the changing environment. Previous reports indicated that Ca 2+ participated in plant gravity response. However, present information on the functions of Ca 2+ in plant gravitropism was obtained mainly on coleoptiles, hypocotyls, and petioles, little is known about the dynamic changes of Ca 2+ during root gravitropism. In the present study, the transgenic Arabidopsis thaliana R-GECO1 was placed horizontally and subsequently vertically on a refitted Leica SP8 laser scanning confocal microscopy with a vertical stage. Real-time observations indicated that gravistimulation induced not only an increase in the Ca 2+ concentration, but also an accelerated occurrence of Ca 2+ sparks in the root cap, especially in the lower side of the lateral root cap, indicating a strong tie between Ca 2+ dynamics and gravistimulation during the early stage of root gravity response.