LytM Fusion with SH3b-Like Domain Expands Its Activity to Physiological Conditions

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Staphylococcus aureus remains one of the most common and at the same time the most dangerous bacteria. The spreading antibiotic resistance calls for intensification of research on staphylococcal physiology and development of new strategies for combating this threatening pathogen. We have engineered new chimeric enzymes comprising the enzymatically active domain (EAD) of autolysin LytM from S. aureus and the cell wall binding domain (CBD) from bacteriocin lysostaphin. They display potent activity in extended environmental conditions. Our results exemplify the possibility of exploring autolytic enzymes in engineering lysins with desired features. Moreover, they suggest a possible mechanism of autolysin physiological activity regulation by local ionic environments in the cell wall.

Related collections

Most cited references 43

Record: found
Abstract: found
Article: not found

Predictors of mortality in Staphylococcus aureus Bacteremia.

Sebastian van Hal, Slade Jensen, Vikram Vaska … (2012)

Staphylococcus aureus bacteremia (SAB) is an important infection with an incidence rate ranging from 20 to 50 cases/100,000 population per year. Between 10% and 30% of these patients will die from SAB. Comparatively, this accounts for a greater number of deaths than for AIDS, tuberculosis, and viral hepatitis combined. Multiple factors influence outcomes for SAB patients. The most consistent predictor of mortality is age, with older patients being twice as likely to die. Except for the presence of comorbidities, the impacts of other host factors, including gender, ethnicity, socioeconomic status, and immune status, are unclear. Pathogen-host interactions, especially the presence of shock and the source of SAB, are strong predictors of outcomes. Although antibiotic resistance may be associated with increased mortality, questions remain as to whether this reflects pathogen-specific factors or poorer responses to antibiotic therapy, namely, vancomycin. Optimal management relies on starting appropriate antibiotics in a timely fashion, resulting in improved outcomes for certain patient subgroups. The roles of surgery and infectious disease consultations require further study. Although the rate of mortality from SAB is declining, it remains high. Future international collaborative studies are required to tease out the relative contributions of various factors to mortality, which would enable the optimization of SAB management and patient outcomes.

0 comments Cited 313 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

Anton Bryksin, Ichiro Matsumura (2010)

Here we describe a straightforward, efficient, and reliable way to clone an insert of choice into a plasmid of choice without restriction endonucleases or T4 DNA ligase. Chimeric primers containing plasmid sequence at the 5' ends and insert sequence at the 3' ends were used to PCR-amplify insertion sequences of various sizes, namely the genes for GFP (gfp), beta-d-glucuronidase (gusA), and beta-galactosidase (lacZ), as well as the entire luxABCDE operon. These inserts were employed as mega-primers in a second PCR with a circular plasmid template. The original plasmid templates were then destroyed in restriction digests with DpnI, and the overlap extension PCR products were used to transform competent Escherichia coli cells. Phusion DNA polymerase was used for the amplification and fusion reactions, so both reactions were easy to monitor and optimize.

0 comments Cited 226 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

MEROPS: the peptidase database

Dominic Tolle, Neil D. Rawlings, Fraser R. Morton … (2008)

Peptidases (proteolytic enzymes or proteases), their substrates and inhibitors are of great relevance to biology, medicine and biotechnology. The MEROPS database (http://merops.sanger.ac.uk) aims to fulfil the need for an integrated source of information about these. The organizational principle of the database is a hierarchical classification in which homologous sets of peptidases and protein inhibitors are grouped into protein species, which are grouped into families and in turn grouped into clans. Important additions to the database include newly written, concise text annotations for peptidase clans and the small molecule inhibitors that are outside the scope of the standard classification; displays to show peptidase specificity compiled from our collection of known substrate cleavages; tables of peptidase–inhibitor interactions; and dynamically generated alignments of representatives of each protein species at the family level. New ways to compare peptidase and inhibitor complements between any two organisms whose genomes have been completely sequenced, or between different strains or subspecies of the same organism, have been devised.

0 comments Cited 123 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Microb Drug Resist

Journal ID (iso-abbrev): Microb. Drug Resist

Journal ID (publisher-id): mdr

Title: Microbial Drug Resistance

Publisher: Mary Ann Liebert, Inc. (140 Huguenot Street, 3rd FloorNew Rochelle, NY 10801USA )

ISSN (Print): 1076-6294

ISSN (Electronic): 1931-8448

Publication date (Print): 01 September 2016

Publication date (Electronic): 01 September 2016

Publication date PMC-release: 01 September 2016

Volume: 22

Issue: 6

Pages: 461-469

Affiliations

[1]International Institute of Molecular and Cell Biology in Warsaw , Warsaw, Poland.

Author notes

Address correspondence to: Izabela Sabała, PhD, International Institute of Molecular and Cell Biology in Warsaw Ks. Trojdena 4, Warsaw 02-109, Poland

E-mail: izabela@ 123456iimcb.gov.pl

Article

Publisher ID: 10.1089/mdr.2016.0053

DOI: 10.1089/mdr.2016.0053

PMC ID: 5036312

PubMed ID: 27351490

SO-VID: 5f93fa02-3567-47b4-816c-7aecd048ce1c

License:

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License ( http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

LytM Fusion with SH3b-Like Domain Expands Its Activity to Physiological Conditions

Read this article at

Abstract

Related collections

Fandom activity

Most cited references 43

Predictors of mortality in Staphylococcus aureus Bacteremia.

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

MEROPS: the peptidase database

Author and article information

Journal

Affiliations

Author notes

Article

History

Page count

Categories

Comments

Comment on this article

Similar content 269

Cited by 16

Most referenced authors 669