For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
9
views
19
references
 Top references
cited by
2
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      97
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Isolation of Escherichia coli carrying the bla CTX-M-1 and qnrS1 genes from reproductive organs of broiler breeders and internal contents of hatching eggs

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          This study aimed to characterize two third-generation cephalosporins- and quinolone-resistant Escherichia coli (TGCs- and Q-R- Ec) isolates recovered from the ovaries of a broiler breeder flock and the internal contents of hatching eggs produced by the broiler breeder flock. Clonal relatedness was determined by multilocus sequence typing (MLST). The isolates displayed the same multidrug resistance profile, with resistance to ampicillin, ticarcillin, piperacillin, cefazollin, cephalothin, cefotaxime, nalidixic acid, tetracycline and sulfonamides. Double disk synergy test demonstrated that the two isolates presented an ESBL phenotype. PCR and sequencing results showed that both the isolates harbored the bla CTX-M-1 and qnrS1 genes. MLST revealed a novel allele combination, designated as ST461, in these isolates. This study would contribute to the molecular epidemiological understanding of TGCs- and/or Q-R- Ec.

          Related collections

          Most cited references19

          • Record: found
          • Abstract: found
          • Article: found
          Is Open Access

          Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains

          Françoise Jaureguy, Luce Landraud, Virginie Passet … (2008)
          Background Extraintestinal pathogenic Escherichia coli (ExPEC) strains represent a huge public health burden. Knowledge of their clonal diversity and of the association of clones with genomic content and clinical features is a prerequisite to recognize strains with a high invasive potential. In order to provide an unbiased view of the diversity of E. coli strains responsible for bacteremia, we studied 161 consecutive isolates from patients with positive blood culture obtained during one year in two French university hospitals. We collected precise clinical information, multilocus sequence typing (MLST) data and virulence gene content for all isolates. A subset representative of the clonal diversity was subjected to comparative genomic hybridization (CGH) using 2,324 amplicons from the flexible gene pool of E. coli. Results Recombination-insensitive phylogenetic analysis of MLST data in combination with the ECOR collection revealed that bacteremic E. coli isolates were highly diverse and distributed into five major lineages, corresponding to the classical E. coli phylogroups (A+B1, B2, D and E) and group F, which comprises strains previously assigned to D. Compared to other strains of phylogenetic group B2, strains belonging to MLST-derived clonal complexes (CCs) CC1 and CC4 were associated (P < 0.05) with a urinary origin. In contrast, no CC appeared associated with severe sepsis or unfavorable outcome of the bacteremia. CGH analysis revealed genomic characteristics of the distinct CCs and identified genomic regions associated with CC1 and/or CC4. Conclusion Our results demonstrate that human bacteremia strains distribute over the entire span of E. coli phylogenetic diversity and that CCs represent important phylogenetic units for pathogenesis and comparative genomics.
          Article 0 comments Cited 131 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Plasmid-mediated quinolone resistance.

            George Jacoby, Jacob Strahilevitz, David Hooper (2014)
            Three mechanisms for plasmid-mediated quinolone resistance (PMQR) have been discovered since 1998. Plasmid genes qnrA, qnrB, qnrC, qnrD, qnrS, and qnrVC code for proteins of the pentapeptide repeat family that protects DNA gyrase and topoisomerase IV from quinolone inhibition. The qnr genes appear to have been acquired from chromosomal genes in aquatic bacteria, are usually associated with mobilizing or transposable elements on plasmids, and are often incorporated into sul1-type integrons. The second plasmid-mediated mechanism involves acetylation of quinolones with an appropriate amino nitrogen target by a variant of the common aminoglycoside acetyltransferase AAC(6')-Ib. The third mechanism is enhanced efflux produced by plasmid genes for pumps QepAB and OqxAB. PMQR has been found in clinical and environmental isolates around the world and appears to be spreading. The plasmid-mediated mechanisms provide only low-level resistance that by itself does not exceed the clinical breakpoint for susceptibility but nonetheless facilitates selection of higher-level resistance and makes infection by pathogens containing PMQR harder to treat.
            Article 0 comments Cited 109 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              The CTX-M conundrum: dissemination of plasmids and Escherichia coli clones.

              Umaer Naseer, Arnfinn Sundsfjord (2011)
              The ongoing global spread and increased prevalence of CTX-M-type extended-spectrum β-lactamases in Enterobacteriaceae is of great concern. The successful distribution of CTX-M enzymes mainly involves Escherichia coli causing systemic as well as urinary tract infections in patients worldwide. CTX-M expression is often associated with coresistance that critically reduces treatments options. The mobilization of bla(CTX-M) genes from their original chromosomal position in various Kluyvera species has been facilitated by mobile genetic elements such as ISEcp1 or ISCR1. Molecular epidemiological studies have revealed a thriving linkage of bla(CTX-M) genes to conjugative plasmids and successful bacterial clones. Multireplicon FII plasmids are shown to carry the most widely distributed bla(CTX-M-15) across continents, paving the way for bla(CTX-M-15) into different genetic lineages of E. coli. Dissemination of virulent clones ST131-O25:H4-B2 and ST405-O102:H6-D is now being described worldwide. Importantly, CTX-M-producing strains are uncovering their ability of long-term gastrointestinal colonization often associated with travel to high-prevalent areas. Thus, we are witnessing a global epidemic of bla(CTX-M)-encoding E. coli strains and plasmids, which require serious attention and efficient infection control measures. © Mary Ann Liebert, Inc.
              Article 0 comments Cited 80 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): J Vet Med Sci
                Journal ID (iso-abbrev): J. Vet. Med. Sci
                Journal ID (publisher-id): JVMS
                Title: The Journal of Veterinary Medical Science
                Publisher: The Japanese Society of Veterinary Science
                ISSN (Print): 0916-7250
                ISSN (Electronic): 1347-7439
                Publication date (Electronic): 03 September 2018
                Publication date (Print): October 2018
                Volume: 80
                Issue: 10
                Pages: 1540-1543
                Affiliations
                [1) ]Faculté des Sciences de la Nature et de la vie, Université Abdelhamid Ibn Badis, Mostaganem 27000, Algeria
                [2) ]Laboratoire de Recherche, Santé et Productions Animales, Ecole Nationale Supérieure Vétérinaire, Algiers 16000, Algeria
                [3) ]Laboratoire de Bactériologie Médicale, Institut Pasteur d’Algérie, Algiers 16000, Algeria
                [4) ]Laboratoire de Microbiologie, Hôpital Central de l’Armé, Algiers 16000, Algeria
                [5) ]Ecole Supérieure des Sciences de l’Aliment et des Industries Agro-alimentaires, Algiers 16000, Algeria
                Author notes
                [* ]Correspondence to: Benameur, Q.: qada.benameur@ 123456univ-mosta.dz
                Article
                Publisher ID: 18-0283
                DOI: 10.1292/jvms.18-0283
                PMC ID: 6207526
                PubMed ID: 30175752
                SO-VID: 6013869e-f0cb-48d1-a322-b754cac3ca6b
                Copyright © ©2018 The Japanese Society of Veterinary Science
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/ )

                History
                Date received : 22 May 2018
                Date accepted : 18 August 2018
                Categories
                Subject: Avian Pathology
                Subject: Note

                Keywords: antimicrobial resistance,molecular epidemiology,ovary,pcr,poultry
                Data availability:
                Keywords: antimicrobial resistance, molecular epidemiology, ovary, pcr, poultry

                Comments

                Comment on this article

                scite_

                Similar content97

                See all similar

                Cited by2

                See all cited by

                Most referenced authors395

                See all reference authors