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      Conservation and sustainable use of medicinal plants: problems, progress, and prospects

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          Abstract

          Medicinal plants are globally valuable sources of herbal products, and they are disappearing at a high speed. This article reviews global trends, developments and prospects for the strategies and methodologies concerning the conservation and sustainable use of medicinal plant resources to provide a reliable reference for the conservation and sustainable use of medicinal plants. We emphasized that both conservation strategies (e.g. in situ and ex situ conservation and cultivation practices) and resource management (e.g. good agricultural practices and sustainable use solutions) should be adequately taken into account for the sustainable use of medicinal plant resources. We recommend that biotechnical approaches (e.g. tissue culture, micropropagation, synthetic seed technology, and molecular marker-based approaches) should be applied to improve yield and modify the potency of medicinal plants.

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          The future of biodiversity.

          Recent extinction rates are 100 to 1000 times their pre-human levels in well-known, but taxonomically diverse groups from widely different environments. If all species currently deemed "threatened" become extinct in the next century, then future extinction rates will be 10 times recent rates. Some threatened species will survive the century, but many species not now threatened will succumb. Regions rich in species found only within them (endemics) dominate the global patterns of extinction. Although new technology provides details of habitat losses, estimates of future extinctions are hampered by our limited knowledge of which areas are rich in endemics.
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            Validation of the ITS2 Region as a Novel DNA Barcode for Identifying Medicinal Plant Species

            Background The plant working group of the Consortium for the Barcode of Life recommended the two-locus combination of rbcL + matK as the plant barcode, yet the combination was shown to successfully discriminate among 907 samples from 550 species at the species level with a probability of 72%. The group admits that the two-locus barcode is far from perfect due to the low identification rate, and the search is not over. Methodology/Principal Findings Here, we compared seven candidate DNA barcodes (psbA-trnH, matK, rbcL, rpoC1, ycf5, ITS2, and ITS) from medicinal plant species. Our ranking criteria included PCR amplification efficiency, differential intra- and inter-specific divergences, and the DNA barcoding gap. Our data suggest that the second internal transcribed spacer (ITS2) of nuclear ribosomal DNA represents the most suitable region for DNA barcoding applications. Furthermore, we tested the discrimination ability of ITS2 in more than 6600 plant samples belonging to 4800 species from 753 distinct genera and found that the rate of successful identification with the ITS2 was 92.7% at the species level. Conclusions The ITS2 region can be potentially used as a standard DNA barcode to identify medicinal plants and their closely related species. We also propose that ITS2 can serve as a novel universal barcode for the identification of a broader range of plant taxa.
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              On the relationship between niche and distribution

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                Author and article information

                Contributors
                slchen@icmm.ac.cn
                yh_2003@126.com
                luohongmei1976@163.com
                wuqiongforever@yahoo.com.cn
                Lichunfang_2005@126.com
                andre.steinmetz@crp-sante.lu
                Journal
                Chin Med
                Chin Med
                Chinese Medicine
                BioMed Central (London )
                1749-8546
                30 July 2016
                30 July 2016
                2016
                : 11
                : 37
                Affiliations
                [1 ]Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700 China
                [2 ]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, 100193 China
                [3 ]Shandong Center of Crop Germplasm Resources, Jinan, 250100 China
                [4 ]School of Pharmacy, Guilin Medical University, Guilin, 541004 China
                [5 ]Plant Molecular Biology Laboratory, Centre de Recherche Public-Sante´, 1526 Luxembourg, Luxembourg
                Article
                108
                10.1186/s13020-016-0108-7
                4967523
                27478496
                615a66fd-0766-4e95-a6da-dc5dbb6630c4
                © Chen et al 2016

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 20 April 2015
                : 15 July 2016
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                Review
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                © The Author(s) 2016

                Complementary & Alternative medicine
                Complementary & Alternative medicine

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