For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
20
views
25
references
 Top references
cited by
12
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      11
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning

      brief-report

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Highlights

          • •

            Fabrication of enzyme-carrying polymeric nanofibers by electrospinning.

          • •

            PVA nanofibers structure and size were not affected by the addition of enzyme.

          • •

            The electrospun CGTase/PVA nanofibers show excellent immobilisation efficiency.

          Abstract

          Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film.

          Related collections

          Most cited references25

          • Record: found
          • Abstract: not found
          • Article: not found

          Cyclodextrins and their uses: a review

          E.M.Martin Del Valle (2004)
          Article 0 comments Cited 491 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Immobilised enzymes: carrier-bound or carrier-free?

            J-L Cao, Luuk van Langen, Phillip R. Sheldon (2003)
            Recent advances have been made in the development of carrier-free immobilised enzymes and several criteria established for the selection of immobilised enzymes for biotransformations. The pros and cons of carrier-free versus carrier-bound immobilised enzymes and of each type of carrier-free enzyme are discussed.
            Article 0 comments Cited 112 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Enzyme-carrying polymeric nanofibers prepared via electrospinning for use as unique biocatalysts.

              Hongfei Jia, Guangyu Zhu, Bradley Vugrinovich … (2015)
              Improvement of catalytic efficiency of immobilized enzymes via materials engineering was demonstrated through the preparation of bioactive nanofibers. Bioactive polystyrene (PS) nanofibers with a typical diameter of 120 nm were prepared and examined for catalytic efficiency for biotransformations. The nanofibers were produced by electrospinning functionalized PS, followed by the chemical attachment of a model enzyme, alpha-chymotrypsin. The observed enzyme loading as determined by active site titration was up to 1.4% (wt/wt), corresponding to over 27.4% monolayer coverage of the external surface of nanofibers. The apparent hydrolytic activity of the nanofibrous enzyme in aqueous solutions was over 65% of that of the native enzyme, indicating a high catalytic efficiency as compared to other forms of immobilized enzymes. Furthermore, nanofibrous alpha-chymotrypsin exhibited a much-improved nonaqueous activity that was over 3 orders of magnitude higher than that of its native counterpart suspended in organic solvents including hexane and isooctane. It appeared that the covalent binding also improved the enzyme's stability against structural denaturation, such that the half-life of the nanofibrous enzyme in methanol was 18-fold longer than that of the native enzyme.
              Article 0 comments Cited 72 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                M. Nazli Naim
                Journal
                Journal ID (nlm-ta): Biotechnol Rep (Amst)
                Journal ID (iso-abbrev): Biotechnol Rep (Amst)
                Title: Biotechnology Reports
                Publisher: Elsevier
                ISSN (Electronic): 2215-017X
                Publication date PMC-release: 09 March 2016
                Publication date Collection: June 2016
                Publication date (Electronic): 09 March 2016
                Volume: 10
                Pages: 44-48
                Affiliations
                [a ]Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan
                [b ]Biotechnology Research Institute, Universiti Malaysia Sabah, 88400, Kota Kinabalu, Sabah, Malaysia
                [c ]Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
                [d ]Department of Chemical Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan
                [e ]Faculty of Chemical Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
                [f ]Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan
                Author notes
                [* ]Corresponding author. mohdnazli@ 123456upm.edu.my
                Article
                Publisher ID: S2215-017X(16)30010-8
                DOI: 10.1016/j.btre.2016.03.003
                PMC ID: 5040860
                SO-VID: 618a652a-fa59-49f8-b1e8-6ea0ae090e6d
                Copyright © © 2016 The Authors
                License:

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                Date received : 5 January 2016
                Date revision received : 7 March 2016
                Date accepted : 7 March 2016
                Categories
                Subject: Article

                Keywords: nanofibres,immobilisation,cgtase,electrospinning,cyclodextrins
                Data availability:
                Keywords: nanofibres, immobilisation, cgtase, electrospinning, cyclodextrins

                Comments

                Comment on this article

                scite_

                Similar content11

                See all similar

                Cited by12

                See all cited by

                Most referenced authors190

                See all reference authors