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Determining the limits and confounders for the 2-pentyl furan breath test by gas chromatography/mass spectrometry.

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      Abstract

      Aspergillus fumigatus produces 2-pentyl furan (2-PF), a volatile compound not produced by many other pathogens or normal human metabolism. 2-Pentyl furan has been detected in the breath of patients with invasive aspergillosis (IA) by SPME pre-concentration coupled with CG/MS providing the possibility of an attractive diagnostic test. The limit of detection (LOD) and quantification (LOQ) for peak integration were assessed both statistically and empirically respectively. 2-Pentyl furan was detected from 10 of 45 food stuffs tested. Levels were highest from soymilk (3 of 3 brands), lower from pumpkin, peanuts, rolled oats 2, Ensure Plus, tinned asparagus, tinned beans and a vegetable exact (Marmite). No 2-PF was detectable in anti-fungal medications used to treat IA or commonly used cosmetics tested. There was no difference in 2-PF breath levels between morning and afternoon or fasting and non fasting samples taken from healthy subjects eating a diet without 2-PF rich foods. 2-Pentyl furan levels were present in breath samples immediately after a mouth rinse with soy milk (P<0.001), and in some subjects after ingesting soy milk and rinsing their mouth with water. The breath test for 2-PF can be conducted without an overnight fast or at a specified time provided the mouth has been rinsed 30 min or more from when 2-PF containing products have been ingested.

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      Author and article information

      Affiliations
      [1 ] University of Otago, Department of Pathology, PO Box 4345, Christchurch 8140, New Zealand.
      Journal
      J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.
      Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
      Elsevier BV
      1873-376X
      1570-0232
      Sep 15 2011
      : 879
      : 26
      21880557
      S1570-0232(11)00524-1
      10.1016/j.jchromb.2011.08.010

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