A modular, cost-effective, versatile, open-source operant box solution for long-term miniscope imaging, 3D tracking, and deep learning behavioral analysis

Abstract

In this procedure we have included an open-source method for a customized operant chamber optimized for long-term miniature microscope (miniscope) recordings.

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  The miniscope box is designed to function with custom or typical med-associates style accessories (e.g., houselights, levers, etc.).

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  The majority of parts can be directly purchased which minimizes the need for skilled and time-consuming labor.

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  We include designs and estimated pricing for a single box but it is recommended to build these in larger batches to efficiently utilize bulk ordering of certain components.

Graphical abstract