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      Pervasive genetic structure at different geographic scales in the coral-excavating sponge Cliona vermifera (Hancock, 1867) in the Mexican Pacific

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          Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.

          S. Aljanabi (1997)
          A very simple, fast, universally applicable and reproducible method to extract high quality megabase genomic DNA from different organisms is described. We applied the same method to extract high quality complex genomic DNA from different tissues (wheat, barley, potato, beans, pear and almond leaves as well as fungi, insects and shrimps' fresh tissue) without any modification. The method does not require expensive and environmentally hazardous reagents and equipment. It can be performed even in low technology laboratories. The amount of tissue required by this method is approximately 50-100 mg. The quantity and the quality of the DNA extracted by this method is high enough to perform hundreds of PCR-based reactions and also to be used in other DNA manipulation techniques such as restriction digestion, Southern blot and cloning.
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            IBD (Isolation by Distance): A Program for Analyses of Isolation by Distance

            A Bohonak (2002)
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              Is Open Access

              Ocean Acidification Accelerates Reef Bioerosion

              Max Wisshak, Christine H. L. Schönberg, Armin Form … (2012)
              In the recent discussion how biotic systems may react to ocean acidification caused by the rapid rise in carbon dioxide partial pressure (pCO2) in the marine realm, substantial research is devoted to calcifiers such as stony corals. The antagonistic process – biologically induced carbonate dissolution via bioerosion – has largely been neglected. Unlike skeletal growth, we expect bioerosion by chemical means to be facilitated in a high-CO2 world. This study focuses on one of the most detrimental bioeroders, the sponge Cliona orientalis, which attacks and kills live corals on Australia’s Great Barrier Reef. Experimental exposure to lowered and elevated levels of pCO2 confirms a significant enforcement of the sponges’ bioerosion capacity with increasing pCO2 under more acidic conditions. Considering the substantial contribution of sponges to carbonate bioerosion, this finding implies that tropical reef ecosystems are facing the combined effects of weakened coral calcification and accelerated bioerosion, resulting in critical pressure on the dynamic balance between biogenic carbonate build-up and degradation.
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                Author and article information

                Journal
                Title: Coral Reefs
                Abbreviated Title: Coral Reefs
                Publisher: Springer Nature
                ISSN (Print): 0722-4028
                ISSN (Electronic): 1432-0975
                Publication date Created: September 2015
                Publication date (Print): June 2015
                Volume: 34
                Issue: 3
                Pages: 887-897
                Article
                DOI: 10.1007/s00338-015-1316-9
                SO-VID: 658db238-a42e-4a65-8961-5b46040ebbc9
                Copyright © © 2015
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