The bewildering observation of structural and functional proteins in the body being
encoded by just a small fraction of the human genome has led to the discovery of the
intriguing fact that >80% of the genome is transcribed into a diverse group of non-coding
RNAs (ncRNAs) (ENCODE Project Consortium, 2012). These ncRNAs can be broadly categorized
into small ncRNAs (eg. microRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs),
which are largely involved in controlling gene expression at transcriptional, post-transcriptional
or epigenetic levels (Batista and Chang, 2013). Development of the nervous system
is a complex and tightly regulated process, which involves dynamic and precise control
of gene expression in a spatio- and temporal-specific manner. It is therefore not
surprising that >40% of ncRNAs are found to be specifically expressed in the nervous
system (Derrien et al., 2012). Accumulating evidence suggests that they play critical
role not only during development but also in the adult stage. Dysregulated function
of ncRNA is expected to result in diseases. Emerging evidence suggests that mutation
of lncRNA or a dysregulation of their expressions correlates with a variety of disorders
in the nervous system, including autism spectrum disorder, amyotrophic lateral sclerosis,
Alzheimer's disease, bipolar disorder, Huntington's disease, Parkinson's disease (Li
et al., 2019).
One of the hallmark pathological features of Parkinson's disease (PD) is the progressive
loss of dopaminergic neurons in the substantia nigra. The decline of dopamine levels
in the striatum results in motor dysfunction. While the underlying causes of PD have
not been completely elucidated, events such as aberrant protein aggregation and degradation,
mitochondrial dysfunction, cell death by apoptosis, autophagy and ferroptosis, oxidative
stress, inflammatory responses are thought to contribute to the loss of dopaminergic
neurons. Interestingly, accumulating evidence suggests that lncRNAs play important
roles in some of these processes, and are plausibly implicated in the pathogenesis
of PD. Selvakumar et al. provided a comprehensive review of the involvement of various
microRNAs (miRNAs) in the regulation of apoptosis, autophagy, mitophagy, and neuroinflammation
in PD. Genes that are targeted by these miRNAs include brain-derived neurotrophic
factor, leucine-rich repeat kinase 2, death-associated protein kinase 1, glutamate
transporter 1, and tumor growth factor-β1. Importantly, these miRNAs also represent
potential therapeutic targets and biomarkers for PD. This is exemplified by findings
in an original research article by Bhattacharyya et al., who reported that the level
of miR-128 was significantly decreased in exosomes derived from PD patients. In a
cellular model of PD induced by 6-OHDA, a downregulation of miR-128 level was also
observed. Forced expression of miR-128 was shown to provide neuroprotective effect
by reducing the production of mitochondrial superoxide and inhibiting the activation
of apoptosis-triggering transcription factor FoxO3a. Mechanistically, miR-128 inhibits
both the intrinsic and extrinsic pathways of apoptosis by suppressing the activation
of pro-apoptotic targets of FoxO3a, like FasL and PUMA. Furthermore, miR-128 prevents
neurite shortening and maintains expression of the presynaptic protein Synaptophysin
and the post-synaptic protein PSD-95. Taken together, miR-128 represents a promising
therapeutic target and diagnostic marker for PD.
CNS injuries, such as traumatic brain injury (TBI), subarachnoid hemorrhage (SAH),
intracerebral hemorrhage (ICH), cerebral ischemic stroke, and spinal cord injury (SCI)
are among the leading causes of disability and mortality. Apart from primary insult,
the ensuing secondary damages in CNS injuries often cause more extensive and chronic
impact. Interplay between complex pathological processes such as oxidative stress,
neuroinflammation, apoptosis, and autophagy results in further neuronal cell death
(Ng and Lee, 2019). Recent studies showed that injury of the nervous system is associated
with alterations in the expression profile of ncRNAs and is dependent on the degree
of severity (Zhang and Wang, 2019). In CNS injuries, ncRNAs are implicated in an array
of physiological processes, like angiogenesis, apoptosis, and inflammation via different
regulatory pathways, hence playing an important role in injury-induced secondary brain
damage. Pedrosa et al. reported a general overexpression of miRNAs in the cerebrospinal
fluid (CSF) of patients with acute SAH, which can develop into delayed cerebral ischemia
(DCI). Specifically, exceptionally high levels of hsa-miR-320e and hsa-miR-451a were
observed in patients who developed post-SAH DCI. Some of the upregulated miRNAs were
implicated in the regulation of pathways that control neurological damage in stroke,
angiogenesis, and integrity of blood-brain barrier (BBB). In short, these findings
suggest the potential use of these miRNAs as prognostic and diagnostic biomarkers
in SAH patients.
It is not an uncommon observation that patients, particularly older adults suffer
from varying degree of cognition dysfunction after surgery. Collectively, post-operative
cognitive dysfunction (POCD) refers to varying degree of decline in learning and memory,
executive function, attention, and orientation after anesthesia and surgery. Surgical
trauma and anesthesia are believed to cause BBB damage, neuroinflammation, mitochondrial
dysfunction, oxidative stress, neuronal apoptosis, autophagy, impaired synaptic function,
and loss of neurotrophic support (Lin et al., 2020). Yang et al. presented an overview
of various ncRNAs that are involved in these cellular processes. Notably, some ncRNAs
have been implicated in anesthesia-induced accumulation of Aβ and hyperphosphorylation
of tau, contributing to neuronal cell death and cognitive dysfunction.
Epilepsy is a common neurological condition due to abnormally excessive electrical
activity in the brain, which causes frequent seizures. Prolonged and uncontrolled
seizure may cause brain damages. Accumulating evidence suggests functional significance
of regulatory immune cells in the modulation of epileptogenic processes and seizures
(Yue et al., 2022). Sharifi et al. reported differential expression of lncRNAs that
are associated with regulatory T cells (Tregs) in different forms of epilepsy. These
findings not only support the involvement of Tregs in epilepsy but also the potential
role of these lncRNAs as diagnostic markers for different epileptic conditions.
While ncRNAs are generally deemed to serve regulatory role in gene expression, increasing
evidence suggests that some of them in fact code for small peptides (Wang et al.,
2019). Since lncRNAs are specifically enriched in the brain, they are more likely
to contribute to the exceptionally high population of neuropeptides in the central
nervous system. Mohaupt et al. provided a timely review of the current development
in alternative proteome in the nervous system, highlighting the underrated importance
of alternative neuroproteome encoded by ncRNAs in various neurodegenerative diseases.
In summary, this Research Topic covered different pathological roles of ncRNAs in
the nervous system. This collection of articles is expected to provide insight and
contributes to the rapid development of ncRNAs as targets for therapeutic intervention
and biomarkers in diseases of the nervous system.
Author contributions
AL prepared the first draft of the editorial. EC and CR commented and revised the
manuscript. All authors approved the final version for publication.