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      • Record: found
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      Is Open Access

      Proteomic analysis identifies Stomatin as a biological marker for psychological stress

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          Abstract

          Psychological stress emerges to be a common health burden in the current society for its highly related risk of mental and physical disease outcomes. However, how the quickly-adaptive stress response process connects to the long-observed organismal alterations still remains unclear. Here, we investigated the profile of circulatory extracellular vesicles (EVs) after acute stress (AS) of restraint mice by phenotypic and proteomic analyses. We surprisingly discovered that AS-EVs demonstrated significant changes in size distribution and plasma concentration compared to control group (CN) EVs. AS-EVs were further characterized by various differentially expressed proteins (DEPs) closely associated with biological, metabolic and immune regulations and were functionally important in potentially underlying multiple diseases. Notably, we first identified the lipid raft protein Stomatin as an essential biomarker expressed on the surface of AS-EVs. These findings collectively reveal that EVs are a significant function-related liquid biopsy indicator that mediate circulation alterations impinged by psychological stress, while also supporting the idea that psychological stress-associated EV-stomatin can be used as a biomarker for potentially predicting acute stress responses and monitoring psychological status. Our study will pave an avenue for implementing routine plasma EV-based theranostics in the clinic.

          Highlights

          • •

            Psychological stress induces phenotypic alteration in plasma extracellular vesicles.

          • •

            Proteomic analysis of extracellular vesicles indicates stress-relevant regulations.

          • •

            Stomatin is unveiled as an extracellular vesicle biomarker for psychological stress.

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          Most cited references54

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          Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication

          Mathilde Mathieu, Lorena Martin-Jaular, Gregory Lavieu … (2019)
          The ability of exosomes to transfer cargo from donor to acceptor cells, thereby triggering phenotypic changes in the latter, has generated substantial interest in the scientific community. However, the extent to which exosomes differ from other extracellular vesicles in terms of their biogenesis and functions remains ill-defined. Here, we discuss the current knowledge on the specificities of exosomes and other types of extracellular vesicles, and their roles as important agents of cell-to-cell communication.
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            Exosomes

            D Pegtel, Stephen Gould (2019)
            Exosomes are small, single-membrane, secreted organelles of ∼30 to ∼200 nm in diameter that have the same topology as the cell and are enriched in selected proteins, lipids, nucleic acids, and glycoconjugates. Exosomes contain an array of membrane-associated, high-order oligomeric protein complexes, display pronounced molecular heterogeneity, and are created by budding at both plasma and endosome membranes. Exosome biogenesis is a mechanism of protein quality control, and once released, exosomes have activities as diverse as remodeling the extracellular matrix and transmitting signals and molecules to other cells. This pathway of intercellular vesicle traffic plays important roles in many aspects of human health and disease, including development, immunity, tissue homeostasis, cancer, and neurodegenerative diseases. In addition, viruses co-opt exosome biogenesis pathways both for assembling infectious particles and for establishing host permissiveness. On the basis of these and other properties, exosomes are being developed as therapeutic agents in multiple disease models.
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              Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis

              Laura M. Doyle, Michael Zhuo Wang (2019)
              The use of extracellular vesicles, specifically exosomes, as carriers of biomarkers in extracellular spaces has been well demonstrated. Despite their promising potential, the use of exosomes in the clinical setting is restricted due to the lack of standardization in exosome isolation and analysis methods. The purpose of this review is to not only introduce the different types of extracellular vesicles but also to summarize their differences and similarities, and discuss different methods of exosome isolation and analysis currently used. A thorough understanding of the isolation and analysis methods currently being used could lead to some standardization in the field of exosomal research, allowing the use of exosomes in the clinical setting to become a reality.
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                Author and article information

                Contributors
                Yan Jin
                Bing-Dong Sui
                Fang Jin
                Journal
                Journal ID (nlm-ta): Neurobiol Stress
                Journal ID (iso-abbrev): Neurobiol Stress
                Title: Neurobiology of Stress
                Publisher: Elsevier
                ISSN (Electronic): 2352-2895
                Publication date PMC-release: 02 January 2023
                Publication date Collection: January 2023
                Publication date (Electronic): 02 January 2023
                Volume: 22
                Electronic Location Identifier: 100513
                Affiliations
                [a ]State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China
                [b ]Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China
                [c ]Department of Preventive Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China
                [d ]Military Medical Psychology School, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China
                [e ]Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China
                [f ]Xi'an Institute of Tissue Engineering and Regenerative Medicine, Xi'an, Shaanxi, 710032, China
                Author notes
                [∗ ]Corresponding author. State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, China. yanjin@ 123456fmmu.edu.cn
                [∗∗ ]Corresponding author. bingdong@ 123456fmmu.edu.cn
                [∗∗∗ ]Corresponding author. Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. jinfang@ 123456fmmu.edu.cn
                [1]

                These authors contributed equally to this work.

                Article
                Publisher Item ID: S2352-2895(23)00001-2 Publisher ID: 100513
                DOI: 10.1016/j.ynstr.2023.100513
                PMC ID: 9829922
                PubMed ID: 36636173
                SO-VID: 686699dc-a152-4cfe-8231-5d185a1e1bd7
                Copyright © © 2023 The Authors
                License:

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                Date received : 7 August 2022
                Date revision received : 24 December 2022
                Date accepted : 2 January 2023
                Categories
                Subject: Original Research Article

                Keywords: psychological stress,extracellular vesicles,proteomic analysis,stomatin,plasma
                Data availability:
                Keywords: psychological stress, extracellular vesicles, proteomic analysis, stomatin, plasma

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