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Abstract
Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1
h pepsin followed by 2 h pancreatin at 37 °C). The antioxidant potential of the BWP
digests was compared by assessing their capacity to scavenge 2,2'-azinobis (3-ethylbenzothiszoline-6-sulphonic
acid) (ABTS(+•)) and hydroxyl ((•)OH) radicals. The 2-h pancreatin digest, which demonstrated
the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration.
Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest
ABTS(+•) scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05).
Fraction VI was most effective in neutralizing (•)OH and was 86 and 24% more efficient
(P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified
Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI)
to be the prominant peptides/amino acid in these fractions.