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      A Single-Cell Transcriptomic Atlas of Human Neocortical Development during Mid-gestation

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          Abstract

          We performed RNA sequencing on 40,000 cells to create a high-resolution single-cell gene expres-sion atlas of developing human cortex, providing the first single-cell characterization of previously uncharacterized cell types, including human sub-plate neurons, comparisons with bulk tissue, and systematic analyses of technical factors. These data permit deconvolution of regulatory networks connecting regulatory elements and transcriptional drivers to single-cell gene expression programs, significantly extending our understanding of human neurogenesis, cortical evolution, and the cellular basis of neuropsychiatric disease. We tie cell-cycle progression with early cell fate decisions during neurogenesis, demonstrating that differentiation occurs on a transcriptomic continuum; rather than only expressing a few transcription factors that drive cell fates, differentiating cells express broad, mixed cell-type transcriptomes before telophase. By mapping neuropsychiatric disease genes to cell types, we implicate dysregulation of specific cell types in ASD, ID, and epilepsy. We developed CoDEx, an online portal to facilitate data access and browsing. An extensive single-cell catalog of cell types in the mid-gestation human neocortex extends our understanding of early cortical development, including subplate neuron transcriptomes, cell type specific regulatory networks, brain evolution and the cellular basis of neuropsychiatric disease.

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          Author and article information

          Journal
          Neuron
          Neuron
          Elsevier BV
          08966273
          July 2019
          July 2019
          Article
          10.1016/j.neuron.2019.06.011
          6831089
          31303374
          6f35726c-16b3-49ac-a674-1bdbb5a8b235
          © 2019

          https://www.elsevier.com/tdm/userlicense/1.0/

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