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Abstract
Extracellular vehicles have a natural targeting ability and immune tolerance of being
usually applied in drug delivery systems; however, the purification of EVs is complicated
and the production yield was quite low. We developed an artificial cellular mimetic
nanovesicle (NV) with melanoma fragment membrane for the transportation with curcumin
to achieve the anticancer purpose. B16F10 derived NVs were manufactured by the breakdown
of cells using a series of extrusions through cut-off size filters (10 and 5 µm),
and the whole procedure was easy and time-saving. To terminate the suspicion of cancer
metastatic issue, B16F10 cells were treated by 30-min sonication and 1-min UVB exposure
to remove genetic materials before the extrusion. B16F10 derived NV loaded with curcumin
was called NV(S30U1/Cur), and the anticancer effect was evaluated by cell-based viability,
immune, migration, and invasion. The results showed that NVs were manufactured by
passing through 10 and 5 µm filters having an enviable production yield, and the mRNA
amounts were declined within NVs produced by B16F10 cells treated with UVB in a comparison
to the control group. NV(S30U1/Cur) were effectively decreased B1610 cell viability,
and migratory and invasive abilities were also reduced significantly. Besides, CD8+
expression of murine primary lymphocytes was activated with CD4+ reduction by NV(S30U1/Cur)
to stimulate the inherent tumor suppressive capacity in the immune system. Taken together,
we established bioengineered NVs serving as novel cell mimetic nanocarriers to deliver
natural compound for malignant melanoma potential immune chemotherapy. DATA AVAILABILITY
STATEMENT: The data used to support the findings of this study are available from
the corresponding author upon requests.