High Rates of Asymptomatic, Sub-microscopic Plasmodium vivax Infection and Disappearing Plasmodium falciparum Malaria in an Area of Low Transmission in Solomon Islands

Abstract

Introduction

Solomon Islands is intensifying national efforts to achieve malaria elimination. A long history of indoor spraying with residual insecticides, combined recently with distribution of long lasting insecticidal nets and artemether-lumefantrine therapy, has been implemented in Solomon Islands. The impact of these interventions on local endemicity of Plasmodium spp. is unknown.

Methods

In 2012, a cross-sectional survey of 3501 residents of all ages was conducted in Ngella, Central Islands Province, Solomon Islands. Prevalence of Plasmodium falciparum, P. vivax, P. ovale and P. malariae was assessed by quantitative PCR (qPCR) and light microscopy (LM). Presence of gametocytes was determined by reverse transcription quantitative PCR (RT-qPCR).

Results

By qPCR, 468 Plasmodium spp. infections were detected (prevalence = 13.4%; 463 P. vivax, five mixed P. falciparum/P. vivax, no P. ovale or P. malariae) versus 130 by LM (prevalence = 3.7%; 126 P. vivax, three P. falciparum and one P. falciparum/P. vivax). The prevalence of P. vivax infection varied significantly among villages (range 3.0–38.5%, p<0.001) and across age groups (5.3–25.9%, p<0.001). Of 468 P. vivax infections, 72.9% were sub-microscopic, 84.5% afebrile and 60.0% were both sub-microscopic and afebrile. Local residency, low education level of the household head and living in a household with at least one other P. vivax infected individual increased the risk of P. vivax infection. Overall, 23.5% of P. vivax infections had concurrent gametocytaemia. Of all P. vivax positive samples, 29.2% were polyclonal by MS16 and msp1F3 genotyping. All five P. falciparum infections were detected in residents of the same village, carried the same msp2 allele and four were positive for P. falciparum gametocytes.

Conclusion

P. vivax infection remains endemic in Ngella, with the majority of cases afebrile and below the detection limit of LM. P. falciparum has nearly disappeared, but the risk of re-introductions and outbreaks due to travel to nearby islands with higher malaria endemicity remains.

Author Summary

Solomon Islands, an island nation in the Southwest Pacific that has seen dramatic reductions in malaria transmission over the past 20 years, is aiming for malaria elimination. There is an increasing recognition that a substantial reservoir of asymptomatic and often sub-microscopic Plasmodium spp. infections exists even in low transmission settings. However, the potential role for these infections in sustaining transmission and the difference in response of the two most common malaria parasites, P. vivax and P. falciparum, to intensified control remains unclear. In May-June 2012, we therefore performed a cross-sectional survey of 3501 residents of all ages of Ngella, a low transmission area in Central Islands Province, to assess the prevalence of P. vivax and P. falciparum infection, determine the proportion of sub-microscopic and afebrile infections and evaluate whether gametocytaemic, and thus potentially infectious, individuals are present. Our survey showed a marked epidemiological contrast between P. vivax and P. falciparum. Although prevalence varied significantly among different regions of Ngella, P. vivax remains firmly endemic, with high rates of sub-microscopic, afebrile and genetically diverse infections. The presence of gametocytes among both sub-microscopic and microscopy positive, asymptomatic infections indicates that these infections contribute significantly to sustaining P. vivax transmission. P. falciparum, on the other hand, appears to be more amenable to control interventions. Only five P. falciparum infected individuals were detected, and all were residents of the same village. These infections carried the same msp2 clone. This difference highlights the larger challenge of eliminating P. vivax compared to P. falciparum in areas where they are co-endemic. In particular, the challenge posed by the presence of a large reservoir of silent P. vivax infections will need to be addressed if control of this parasite is to be accelerated and elimination achieved.