Metabonomics is a new technology providing broad information about dynamic metabolic responses in living systems to pathophysiological stimuli or genetic modification. Nuclear magnetic resonance (NMR) spectroscopy is one of the most powerful methods in metabonomics; it is utilized to establish the metabolic profiles of biofluids, and is practically the only method capable of examining intact tissue samples. Experience with the application of metabonomics in eye research is still limited, yet this method provides the possibility of exploring metabolic processes in the eye in vivo. This article presents a brief background to the usefulness of metabonomics, and the possible applications of an NMR-based technique in eye research and clinical practice.