6
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Cry1Ba1-mediated toxicity of transgenic Bergera koenigii and Citrus sinensis to the Asian citrus psyllid Diaphorina citri

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The Asian citrus psyllid, Diaphorina citri, vectors the bacterial causative agent of citrus greening disease, which has severely impacted citrus production on a global scale. As the current repeated application of chemical insecticides is unsustainable for management of this insect and subsequent protection of groves, we investigated the potential use of the bacteria-derived pesticidal protein, Cry1Ba1, when delivered via transgenic citrus plants. Having demonstrated transformation of the Indian curry leaf tree, Bergera koenigii, for Cry1Ba1 expression for use as a trap plant, we produced transgenic plants of Duncan grapefruit, Citrus paridisi, Valencia sweet orange, Citrus sinensis, and Carrizo citrange, C. sinensis x Poncirus trifoliata, for expression of Cry1Ba1. The presence of the cry1ba1 gene, and cry1ba1 transcription were confirmed. Western blot detection of Cry1Ba1 was confirmed in most cases. When compared to those from wild-type plants, leaf discs from transgenic Duncan and Valencia expressing Cry1Ba1 exhibited a “delayed senescence” phenotype, similar to observations made for transgenic B. koenigii. In bioassays, significant reductions in the survival of adult psyllids were noted on transgenic B. koenigii and Valencia sweet orange plants expressing Cry1Ba1, but not on transgenic Duncan grapefruit or Carrizo citrange. In contrast to psyllids fed on wild type plants, the gut epithelium of psyllids fed on transgenic plants was damaged, consistent with the mode of action of Cry1Ba1. These results indicate that the transgenic expression of a bacterial pesticidal protein in B. koenigii and Valencia sweet orange offers a viable option for management of D. citri, that may contribute to solutions that counter citrus greening disease.

          Related collections

          Most cited references69

          • Record: found
          • Abstract: found
          • Article: not found

          Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

          The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data. Copyright 2001 Elsevier Science (USA).
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Nonparametric Estimation from Incomplete Observations

                Bookmark

                Author and article information

                Contributors
                Journal
                Front Insect Sci
                Front Insect Sci
                Front. Insect Sci.
                Frontiers in Insect Science
                Frontiers Media S.A.
                2673-8600
                2673-8600
                24 April 2023
                2023
                : 3
                : 1125987
                Affiliations
                [1] 1 Citrus Research and Education Center, Institute of Food and Agricultural Sciences (IFAS), University of Florida , Lake Alfred, FL, United States
                [2] 2 Entomology and Nematology Department, Institute of Food and Agricultural Sciences (IFAS), University of Florida , Gainesville, FL, United States
                Author notes

                Edited by: Peng He, Guizhou University, China

                Reviewed by: Jeffrey A. Fabrick, Agricultural Research Service (USDA), United States; Xingliang Wang, Nanjing Agricultural University, China

                *Correspondence: Bryony C. Bonning, bbonning@ 123456ufl.edu

                †Present address: Seyed Ali Ravanfar, Research and Development, InnerPlant, Davis, CA, United States; Freddy Ibanez-Carrasco, Department of Entomology, Texas A&M AgriLife Research, Weslaco, TX, United States

                This article was submitted to Insect Molecular Genetics, a section of the journal Frontiers in Insect Science

                Article
                10.3389/finsc.2023.1125987
                10926525
                38469526
                72222b07-ed8d-4424-9fec-47f3ddefdcea
                Copyright © 2023 Orbović, Ravanfar, Achor, Shilts, Ibanez-Carrasco, Banerjee, El-Mohtar, Stelinski and Bonning

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 16 December 2022
                : 06 April 2023
                Page count
                Figures: 3, Tables: 3, Equations: 0, References: 62, Pages: 12, Words: 7022
                Funding
                Funded by: U.S. Department of Agriculture , doi 10.13039/100000199;
                Award ID: 2017-70016-26755
                This project was supported by the Citrus Diseases Research and Extension grants program, Award number 2017-70016-26755 and by the Emergency Citrus Diseases Research and Extension grants program, Award number 2020-70029-33177 from the USDA National Institute of Food and Agriculture.
                Categories
                Insect Science
                Original Research

                genetic transformation,pesticidal protein,bacillus thuringiensis,bt toxin,insect resistant transgenic plants

                Comments

                Comment on this article