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      Immobilized proteins in buffer imaged at molecular resolution by atomic force microscopy

      , , , , , , , ,

      Biophysical Journal

      Elsevier BV

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          Abstract

          Samples of supported planar lipid-protein membranes and actin filaments on mica were imaged by atomic force microscopy (AFM). The samples were fully submerged in buffer at room temperature during imaging. Individual proteins bound to the reconstituted membrane were distinguishable; some structural details could be resolved. Also, surface-induced, self-assembling of actin filaments on mica could be observed. Monomeric subunits were imaged on individual actin filaments. The filaments could be manipulated on or removed from the surface by the tip of the AFM. The process of the decoupling of the filamentous network from the surface upon changing the ionic conditions was imaged in real time.

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          Author and article information

          Journal
          Biophysical Journal
          Biophysical Journal
          Elsevier BV
          00063495
          November 1990
          November 1990
          : 58
          : 5
          : 1251-1258
          Article
          10.1016/S0006-3495(90)82465-6
          1281069
          2291944
          © 1990

          https://www.elsevier.com/tdm/userlicense/1.0/

          https://www.elsevier.com/open-access/userlicense/1.0/

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