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      Viability Prediction of Ricinus cummunis L. Seeds Using Multispectral Imaging

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          Abstract

          The purpose of this study was to highlight the use of multispectral imaging in seed quality testing of castor seeds. Visually, 120 seeds were divided into three classes: yellow, grey and black seeds. Thereafter, images at 19 different wavelengths ranging from 375–970 nm were captured of all the seeds. Mean intensity for each single seed was extracted from the images, and a significant difference between the three colour classes was observed, with the best separation in the near-infrared wavelengths. A specified feature (RegionMSI mean) based on normalized canonical discriminant analysis, were employed and viable seeds were distinguished from dead seeds with 92% accuracy. The same model was tested on a validation set of seeds. These seeds were divided into two groups depending on germination ability, 241 were predicted as viable and expected to germinate and 59 were predicted as dead or non-germinated seeds. This validation of the model resulted in 96% correct classification of the seeds. The results illustrate how multispectral imaging technology can be employed for prediction of viable castor seeds, based on seed coat colour.

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            Seed longevity: survival and maintenance of high germination ability of dry seeds.

            The seed constitutes the main vector of plant propagation and it is a critical development stage with many specificities. Seed longevity is a major challenge for the conservation of plant biodiversity and for crop success. Seeds possess a wide range of systems (protection, detoxification, repair) allowing them to survive in the dry state and to preserve a high germination ability. Therefore, the seed system provides an appropriate model to study longevity and aging.
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              Seed Germination — The Biochemical and Molecular Mechanisms

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                Author and article information

                Journal
                Sensors (Basel)
                Sensors (Basel)
                Sensors (Basel, Switzerland)
                MDPI
                1424-8220
                February 2015
                17 February 2015
                : 15
                : 2
                : 4592-4604
                Affiliations
                [1 ] Department of Agroecology, Science and Technology, Aarhus University, Slagelse 4200, Denmark; E-Mails: santosh.shrestha@ 123456agro.au.dk (S.S.); Lise.deleuran@ 123456agro.au.dk (L.C.D.); Birte.boelt@ 123456agro.au.dk (B.B.); Rene.gislum@ 123456agro.au.dk (R.G.)
                [2 ] Department of Agronomy, Shahrekord University, Shahrekord 88176-53849, Chaharmahal Bakhtiyari, Iran; E-Mails: Nikneshan@ 123456stu.sku.ac.ir (P.N.); tadayyon.sku@ 123456gmail.com (A.T.)
                Author notes

                Academic Editor: Gonzalo Pajares Martinsanz

                [* ]Author to whom correspondence should be addressed; E-Mail: merete.olesen@ 123456agro.au.dk ; Tel.: +45-871-58386.
                Article
                sensors-15-04592
                10.3390/s150204592
                4367427
                25690554
                7760b533-999f-4e8d-a669-de579a150a60
                © 2015 by the authors; licensee MDPI, Basel, Switzerland.

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 22 December 2014
                : 09 February 2015
                Categories
                Article

                Biomedical engineering
                multispectral imaging,castor seed,canonical discriminant analysis (cda),viability,germination

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