Correction: In Vivo Imaging Reveals a Pioneer Wave of Monocyte Recruitment into Mouse Skin Wounds

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Figure S1 is incorrect. Please see the corrected Figure S1 here. Supporting Information Figure S1 GFP+ ECFP- cells were detectable within the skin surrounding the wound. Representative TPLSM pictures of superficial skin layer from MacBlue ×CX3CR1gfp/+ mice at proximity of the wound edge. SHG signal is in blue, GFP signal is in green. (PPTX) Click here for additional data file.

Related collections

Most cited references 1

Record: found
Abstract: found
Article: found

Is Open Access

In Vivo Imaging Reveals a Pioneer Wave of Monocyte Recruitment into Mouse Skin Wounds

Mathieu Rodero, Fabrice Licata, Lucie Poupel … (2014)

The cells of the mononuclear phagocyte system are essential for the correct healing of adult skin wounds, but their specific functions remain ill-defined. The absence of granulation tissue immediately after skin injury makes it challenging to study the role of mononuclear phagocytes at the initiation of this inflammatory stage. To study their recruitment and migratory behavior within the wound bed, we developed a new model for real-time in vivo imaging of the wound, using transgenic mice that express green and cyan fluorescent proteins and specifically target monocytes. Within hours after the scalp injury, monocytes invaded the wound bed. The complete abrogation of this infiltration in monocyte-deficient CCR2−/− mice argues for the involvement of classical monocytes in this process. Monocyte infiltration unexpectedly occurred as early as neutrophil recruitment did and resulted from active release from the bloodstream toward the matrix through microhemorrhages rather than transendothelial migration. Monocytes randomly scouted around the wound bed, progressively slowed down, and stopped. Our approach identified and characterized a rapid and earlier than expected wave of monocyte infiltration and provides a novel framework for investigating the role of these cells during early stages of wound healing.

0 comments Cited 25 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): PLoS One

Journal ID (iso-abbrev): PLoS ONE

Journal ID (publisher-id): plos

Journal ID (pmc): plosone

Title: PLoS ONE

Publisher: Public Library of Science (San Francisco, USA )

ISSN (Electronic): 1932-6203

Publication date Collection: 2014

Publication date (Electronic): 8 December 2014

Volume: 9

Issue: 12

Electronic Location Identifier: e115508

Article

Publisher ID: PONE-D-14-53102

DOI: 10.1371/journal.pone.0115508

PMC ID: 4259483

PubMed ID: 25486013

SO-VID: 7b20f024-66e5-4501-b602-c819893d427d

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Correction: In Vivo Imaging Reveals a Pioneer Wave of Monocyte Recruitment into Mouse Skin Wounds

Read this article at

Abstract

Related collections

PLOS Climate

Most cited references 1

In Vivo Imaging Reveals a Pioneer Wave of Monocyte Recruitment into Mouse Skin Wounds

Author and article information

Journal

Article

History

Page count

Categories

Comments

Comment on this article

Similar content 22

Cited by 1

Most referenced authors 7