Isolation and characterization of bacteriophages against virulent  Aeromonas hydrophila

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bacteriophages have been attracting increasing attention as a potential alternative to antibiotics against bacterial infections.

Results

Five phages against pathogenic A. hydrophila, named N21, W3, G65, Y71 and Y81, were isolated. Morphological analysis by transmission electron microscopy revealed that phages N21, W3 and G65 belong to the family Myoviridae, while Y71 and Y81 belong to the Podoviridae. These phages were found to have broad host spectra, short latent periods and normal burst sizes. They were sensitive to high temperature but had a wide adaptability to the pH. In addition, the phages G65 and Y81 showed considerable bacterial killing effect and potential in preventing formation of A. hydrophila biofilm; and the phages G65, W3 and N21 were able to scavenge mature biofilm effectively. Phage treatments applied to the pathogenic A. hydrophila in mice model resulted in a significantly decreased bacterial loads in tissues.

Conclusions

Five A. hydrophila phages were isolated with broad host ranges, low latent periods, and wide pH and thermal tolerance. And the phages exhibited varying abilities in controlling A. hydrophila infection. This work presents promising data supporting the future use of phage therapy.

Related collections

Most cited references 55

Record: found
Abstract: found
Article: not found

Pros and cons of phage therapy.

Catherine Loc-Carrillo, Stephen Abedon (2011)

Many publications list advantages and disadvantages associated with phage therapy, which is the use of bacterial viruses to combat populations of nuisance or pathogenic bacteria. The goal of this commentary is to discuss many of those issues in a single location. In terms of "Pros," for example, phages can be bactericidal, can increase in number over the course of treatment, tend to only minimally disrupt normal flora, are equally effective against antibiotic-sensitive and antibiotic-resistant bacteria, often are easily discovered, seem to be capable of disrupting bacterial biofilms, and can have low inherent toxicities. In addition to these assets, we consider aspects of phage therapy that can contribute to its safety, economics, or convenience, but in ways that are perhaps less essential to the phage potential to combat bacteria. For example, autonomous phage transfer between animals during veterinary application could provide convenience or economic advantages by decreasing the need for repeated phage application, but is not necessarily crucial to therapeutic success. We also consider possible disadvantages to phage use as antibacterial agents. These "Cons," however, tend to be relatively minor.

0 comments Cited 319 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Global trends in antimicrobial resistance in animals in low- and middle-income countries

Thomas P Van Boeckel, João Pires, Reshma Silvester … (2019)

The global scale-up in demand for animal protein is the most notable dietary trend of our time. Antimicrobial consumption in animals is threefold that of humans and has enabled large-scale animal protein production. The consequences for the development of antimicrobial resistance in animals have received comparatively less attention than in humans. We analyzed 901 point prevalence surveys of pathogens in developing countries to map resistance in animals. China and India represented the largest hotspots of resistance, with new hotspots emerging in Brazil and Kenya. From 2000 to 2018, the proportion of antimicrobials showing resistance above 50% increased from 0.15 to 0.41 in chickens and from 0.13 to 0.34 in pigs. Escalating resistance in animals is anticipated to have important consequences for animal health and, eventually, for human health.

0 comments Cited 289 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance.

Thien-Fah Mah, Betsey Pitts, Brett Pellock … (2003)

Biofilms are surface-attached microbial communities with characteristic architecture and phenotypic and biochemical properties distinct from their free-swimming, planktonic counterparts. One of the best-known of these biofilm-specific properties is the development of antibiotic resistance that can be up to 1,000-fold greater than planktonic cells. We report a genetic determinant of this high-level resistance in the Gram-negative opportunistic pathogen, Pseudomonas aeruginosa. We have identified a mutant of P. aeruginosa that, while still capable of forming biofilms with the characteristic P. aeruginosa architecture, does not develop high-level biofilm-specific resistance to three different classes of antibiotics. The locus identified in our screen, ndvB, is required for the synthesis of periplasmic glucans. Our discovery that these periplasmic glucans interact physically with tobramycin suggests that these glucose polymers may prevent antibiotics from reaching their sites of action by sequestering these antimicrobial agents in the periplasm. Our results indicate that biofilms themselves are not simply a diffusion barrier to these antibiotics, but rather that bacteria within these microbial communities employ distinct mechanisms to resist the action of antimicrobial agents.

0 comments Cited 263 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Yongjie Liu:

ORCID: http://orcid.org/0000-0001-7301-9501

liuyongjie@njau.edu.cn

Journal

Journal ID (nlm-ta): BMC Microbiol

Journal ID (iso-abbrev): BMC Microbiol

Title: BMC Microbiology

Publisher: BioMed Central (London )

ISSN (Electronic): 1471-2180

Publication date (Electronic): 1 June 2020

Publication date PMC-release: 1 June 2020

Publication date Collection: 2020

Volume: 20

Electronic Location Identifier: 141

Affiliations

[1 ]GRID grid.27871.3b, ISNI 0000 0000 9750 7019, Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, , Nanjing Agricultural University, ; Nanjing, 210095 China

[2 ]Sucheng District Animal Husbandry and Veterinary Station, Suqian, 223800 China

Author information

Yongjie Liu http://orcid.org/0000-0001-7301-9501

Article

Publisher ID: 1811

DOI: 10.1186/s12866-020-01811-w

PMC ID: 7268745

PubMed ID: 32487015

SO-VID: 7b75b6a3-cf18-41a8-bb5b-d9e2f7e4bfce

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 14 January 2020

Date accepted : 4 May 2020

Funding

Funded by: National Key R & D Program of China

Award ID: 2019YFC1605402

Award Recipient : Yuhao Dong

Funded by: Three New Aquatic Projects in Jiangsu Province

Award ID: D2017-3-1

Award Recipient : Yongjie Liu

Funded by: Independent Innovation Fund of Agricultural Science and Technology in Jiangsu Province

Award ID: CX(17)2027, CX(18)2011

Award Recipient : Yongjie Liu

Funded by: Priority Academic Program Development of Jiangsu Higher Education Institutions

Award ID: PAPD

Award Recipient : Yongjie Liu

Custom metadata

ScienceOpen disciplines: Microbiology & Virology

Keywords: aeromonas hydrophila,bacteriophage,biological characteristics,biofilm,phage therapy

Data availability:

ScienceOpen disciplines: Microbiology & Virology

Keywords: aeromonas hydrophila, bacteriophage, biological characteristics, biofilm, phage therapy

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Read this article at

Abstract

Background

Results

Conclusions

Related collections

Bacterial vaginosis and biofilm

Most cited references 55

Pros and cons of phage therapy.

Global trends in antimicrobial resistance in animals in low- and middle-income countries

A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance.

Author and article information

Contributors

Journal

Affiliations

Author information

Article

History

Funding

Categories

Custom metadata

Comments

Comment on this article

Similar content 429

Cited by 23

Most referenced authors 670