113
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation.

      1 , , ,
      Biochemical and biophysical research communications
      Elsevier BV

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that uses only one type of enzyme. One of the characteristics of the LAMP method is its ability to synthesize extremely large amount of DNA. Accordingly, a large amount of by-product, pyrophosphate ion, is produced, yielding white precipitate of magnesium pyrophosphate in the reaction mixture. Judging the presence or absence of this white precipitate allows easy distinction of whether nucleic acid was amplified by the LAMP method. Since an increase in the turbidity of the reaction mixture according to the production of precipitate correlates with the amount of DNA synthesized, real-time monitoring of the LAMP reaction was achieved by real-time measurement of turbidity.

          Related collections

          Author and article information

          Journal
          Biochem Biophys Res Commun
          Biochemical and biophysical research communications
          Elsevier BV
          0006-291X
          0006-291X
          Nov 23 2001
          : 289
          : 1
          Affiliations
          [1 ] Eiken Chemical Company, Ltd., 1381-3 Shimoishigami, Ohtawara, Tochigi 324-0036, Japan. yasuyoshi_mori@eiken.co.jp
          Article
          S0006-291X(01)95921-2
          10.1006/bbrc.2001.5921
          11708792
          7bd93622-a445-413a-a504-88275ed22115
          Copyright 2001 Academic Press.
          History

          Comments

          Comment on this article