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      Methods for the diagnosis of bacterial fish diseases

      , 1

      Marine Life Science & Technology

      Springer

      Culturing, Phenotyping, Serology, Molecular methods, Rapid techniques

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          Abstract

          The diagnosis of bacterial fish diseases has progressed from traditional culture-dependent methods involving the recovery of pathogens on agar-containing media and identification by examination of phenotypic traits. Newer approaches centre on culture-independent approaches. A problem with culturing is that it lacks sensitivity, tends to be slow, and its success depends on the composition of the media and incubation conditions employed. In contrast, culture-independent methods, now centring on molecular methods, are highly specific and sensitive. This raises an important issue that detection of very low numbers of bacterial cells does not necessarily imply the presence of clinical disease. Positivity could reflect background populations of the pathogen that may be present in the aquatic environment.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 November 2019
          11 November 2019
          : 1
          : 1
          : 41-49
          Affiliations
          1Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK
          Author notes
          *Corresponding author: Brian Austin, E-mail: baustin5851@ 123456gmail.com
          Article
          s42995-019-00002-5
          10.1007/s42995-019-00002-5
          © 2019 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
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