This study assessed the effectiveness of Quality Academy Teams Training, a team-based process improvement program at Mayo Clinic. The study population consisted of employees who attended the course in 2008 (n = 103). A pretest-posttest design was used to assess learning by participants of the course, and gain score analysis was conducted using paired t test procedures. Electronic surveys were sent to participants 90 days following completion of the course to assess self-reported application of skills and process improvement tools in the work setting. The mean overall score (n = 99) for the posttest was 68%, which was a significant improvement from the pretest mean of 48% (P < .001). Survey results showed that respondents (n = 58) increased their use of 36 specific process improvement tools on the job after attending the training (P < .001). Other health care institutions may benefit from the implementation of quality-related training programs that teach employees to use process improvement tools and methods.