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      Molecular diversity analysis of Tetradium ruticarpum ( WuZhuYu) in China based on inter-primer binding site (iPBS) markers and inter-simple sequence repeat (ISSR) markers

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          Abstract

          Wu zhu yu”, which is obtained from the dried unripe fruits of Tetradium ruticarpum (A. Jussieu) T. G. Hartley, has been used as a traditional Chinese medicine for treatment of headaches, abdominal colic, and hypertension for thousands of years. The present study was designed to assess the molecular genetic diversity among 25 collected accessions of T. ruticarpum ( Wu zhu yu in Chinese) from different areas of China, based on inter-primer binding site (iPBS) markers and inter-simple sequence repeat (ISSR) markers. Thirteen ISSR primers generated 151 amplification bands, of which 130 were polymorphic. Out of 165 bands that were amplified using 10 iPBS primers, 152 were polymorphic. The iPBS markers displayed a higher proportion of polymorphic loci (PPL = 92.5%) than the ISSR markers (PPL = 84.9%). The results showed that T. ruticarpum possessed high loci polymorphism and genetic differentiation occurred in this plant. The combined data of iPBS and ISSR markers scored on 25 accessions produced five clusters that approximately matched the geographic distribution of the species. The results indicated that both iPBS and ISSR markers were reliable and effective tools for analyzing the genetic diversity in T. ruticarpum.

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          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 January 2018
          : 16
          : 1
          : 1-9
          Affiliations
          Department of Resources Science of Traditional Chinese Medicines, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
          Author notes
          *Corresponding author: QIN Min-Jian, Tel: 86-25-86185130, Fax: 86-25-85301528, E-mail: minjianqin@ 123456163.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(18)30024-4
          10.1016/S1875-5364(18)30024-4
          Copyright © 2018 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: Chinese Herbal Medicine Seeds and Seedlings Planting (breeding) Standard Platform Topics
          Award ID: 2012ZX09304006
          This work was supported by the National Science and Technology Major Projects for “Major New Drugs Innovation and Development” and the “Chinese Herbal Medicine Seeds and Seedlings Planting (breeding) Standard Platform Topics” (No. 2012ZX09304006).
          Categories
          Research Articles

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