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      Biosynthesis of ( R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake

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          Abstract

          Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is ( R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of ( R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of ( R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of ( R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.

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          Consolidated bioprocessing of cellulosic biomass: an update.

          Lee R Lynd, Willem H. van Zyl, John E. Mcbride … (2005)
          Biologically mediated processes seem promising for energy conversion, in particular for the conversion of lignocellulosic biomass into fuels. Although processes featuring a step dedicated to the production of cellulase enzymes have been the focus of most research efforts to date, consolidated bioprocessing (CBP)--featuring cellulase production, cellulose hydrolysis and fermentation in one step--is an alternative approach with outstanding potential. Progress in developing CBP-enabling microorganisms is being made through two strategies: engineering naturally occurring cellulolytic microorganisms to improve product-related properties, such as yield and titer, and engineering non-cellulolytic organisms that exhibit high product yields and titers to express a heterologous cellulase system enabling cellulose utilization. Recent studies of the fundamental principles of microbial cellulose utilization support the feasibility of CBP.
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            A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

            R S Sikorski, P Hieter, Daniel Ho (1989)
            A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae. Transplacement vectors were constructed and used to derive yeast strains containing nonreverting his3, trp1, leu2 and ura3 mutations. A set of YCp and YIp vectors (pRS series) was then made based on the backbone of the multipurpose plasmid pBLUESCRIPT. These pRS vectors are all uniform in structure and differ only in the yeast selectable marker gene used (HIS3, TRP1, LEU2 and URA3). They possess all of the attributes of pBLUESCRIPT and several yeast-specific features as well. Using a pRS vector, one can perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
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              The population genetics of commensal Escherichia coli.

              Olivier Tenaillon, David Skurnik, Bertrand Picard … (2010)
              The primary habitat of Escherichia coli is the vertebrate gut, where it is the predominant aerobic organism, living in symbiosis with its host. Despite the occurrence of recombination events, the population structure is predominantly clonal, allowing the delineation of major phylogenetic groups. The genetic structure of commensal E. coli is shaped by multiple host and environmental factors, and the determinants involved in the virulence of the bacteria may in fact reflect adaptation to commensal habitats. A better characterization of the commensal niche is necessary to understand how a useful commensal can become a harmful pathogen. In this Review we describe the population structure of commensal E. coli, the factors involved in the spread of different strains, how the bacteria can adapt to different niches and how a commensal lifestyle can evolve into a pathogenic one.
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                Author and article information

                Journal
                Journal ID (nlm-ta): J Microbiol Biotechnol
                Journal ID (iso-abbrev): J Microbiol Biotechnol
                Title: Journal of Microbiology and Biotechnology
                Publisher: Korean Society for Microbiology and Biotechnology
                ISSN (Print): 1017-7825
                ISSN (Electronic): 1738-8872
                Publication date (Print): 28 February 2020
                Publication date (Electronic): 17 February 2020
                Volume: 30
                Issue: 2
                Pages: 296-305
                Affiliations
                [1 ]School of Life Science and Biotechnology, Kyungpook National University, Daegu 4566, Republic of Korea
                [2 ]School of Life Science, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea
                [3 ]Department of Biotechnology, The University of Tokyo 1-1-1 Yayoi, Bunkyo-ku Tokyo 11-8657 Japan
                Author notes
                [* ] Corresponding authors J.G.K. Phone: +82-53-950-5379 Fax: +82-53-955-5379 E-mail: kimjg@ 123456knu.ac.kr
                [†]

                These authors contributed equally to this work.

                Article
                Publisher ID: JMB-30-2-296
                DOI: 10.4014/jmb.2001.01049
                PMC ID: 9728333
                PubMed ID: 32120462
                SO-VID: 839e0eee-6257-43ae-8458-9d9e63c0460e
                Copyright © Copyright©2020 by The Korean Society for Microbiology and Biotechnology
                License:

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.

                History
                Date received : 29 January 2020
                Date revision received : 04 February 2020
                Date accepted : 16 February 2020
                Categories
                Subject: Research article
                Subject: Molecular and Cellular Microbiology (MCM)
                Subject: Biochemistry and Physiology

                Keywords: (r)-(-)-1-octen-3-ol,tricholoma matsutake,lipoxygenase,hydroperoxide lyase,saccharomyces cerevisiae
                Data availability:
                Keywords: (r)-(-)-1-octen-3-ol, tricholoma matsutake, lipoxygenase, hydroperoxide lyase, saccharomyces cerevisiae

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