We investigate the effect of early chemical freeze-out on radial flow, elliptic flow and HBT radii by using a fully three dimensional hydrodynamic model. When we take account of the early chemical freeze-out, the space-time evolution of temperature in the hadron phase is considerably different from the conventional model in which chemical equilibrium is always assumed. As a result, we find that radial and elliptic flows are suppressed and that the lifetime and the spatial size of the fluid are reduced. We analyze the p_t spectrum, the differential elliptic flow, and the HBT radii at the RHIC energy by using hydrodynamics with chemically non-equilibrium equation of state.