For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
7
views
41
references
 Top references
cited by
1
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      149
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells

      research-article

      Read this article at

      SciELOPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Abstract: Modified formulations of calcium silicate repair materials with additives have been developed to enhance handling, consistency, biocompatibility and bioactivity. Considering the relevance of osteoblastic cell response to mineralized tissue repair, human osteoblastic cells (Saos-2 cells overexpressing BMP-2) were exposed to mineral trioxide aggregate (MTA) (with calcium tungstate - CaWO4), MTA HP Repair, Bio-C Repair and Bio-C Pulpo. Cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR), and cell death, by flow cytometry. Gene expression of bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX-2), and alkaline phosphatase (ALP) osteogenic markers were evaluated by real-time polymerase chain reaction (RT-qPCR). ALP activity and alizarin red staining (ARS) were used to detect mineralization nodule deposition. Bioactive cements presented no cytotoxic effect, and did not induce apoptosis at the higher dilution (1:12). MTA, Bio-C Repair and Bio-C Pulpo exhibited higher ALP activity than the control group (P < 0.05) after 7 days. MTA, MTA HP and Bio-C Pulpo affected the formation of mineralized nodules (p < 0.05). Exposure to all cement extracts for 1 day increased BMP-2 gene expression. RUNX-2 mRNA was greater in MTA, MTA HP and Bio-C Repair. MTA, MTA HP and Bio-C Pulpo increased the ALP mRNA expression, compared with BMP-2 unexposed cells (P < 0.05). Calcium silicate cements showed osteogenic potential and biocompatibility in Saos-2 cells transfected BMP-2, and increased the mRNA expression of BMP-2, RUNX-2, and ALP osteogenic markers in the BMP-2 transfected system, thereby promoting a cellular response to undertake the mineralized tissue repair.

          Related collections

          Most cited references41

          • Record: found
          • Abstract: found
          • Article: not found

          Regulation of bone development and extracellular matrix protein genes by RUNX2.

          Toshihisa Komori (2010)
          RUNX2 is a multifunctional transcription factor that controls skeletal development by regulating the differentiation of chondrocytes and osteoblasts and the expression of many extracellular matrix protein genes during chondrocyte and osteoblast differentiation. This transcription factor plays a major role at the late stage of chondrocyte differentiation: it is required for chondrocyte maturation and regulates Col10a1 expression in hypertrophic chondrocytes and the expression of Spp1, Ibsp, and Mmp13 in terminal hypertrophic chondrocytes. It is essential for the commitment of pluripotent mesenchymal cells to the osteoblast lineage. During osteoblast differentiation, RUNX2 upregulates the expression of bone matrix protein genes including Col1a1, Spp1, Ibsp, Bglap, and Fn1 in vitro and activates many promoters including those of Col1a1, Col1a2, Spp1, Bglap, and Mmp13. However, overexpression of Runx2 inhibits osteoblast maturation and reduces Col1a1 and Bglap expression. The inhibition of RUNX2 in mature osteoblasts does not reduce the expression of Col1a1 and Bglap in mice. Thus, RUNX2 directs pluripotent mesenchymal cells to the osteoblast lineage, triggers the expression of major bone matrix protein genes, and keeps the osteoblasts in an immature stage, but does not play a major role in the maintenance of the expression of Col1a1 or Bglap in mature osteoblasts. During bone development, RUNX2 induces osteoblast differentiation and increases the number of immature osteoblasts, which form immature bone, whereas Runx2 expression has to be downregulated for differentiation into mature osteoblasts, which form mature bone. During dentinogenesis, Runx2 expression is downregulated, and RUNX2 inhibits the terminal differentiation of odontoblasts.
          Article 0 comments Cited 232 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Sealing ability of a mineral trioxide aggregate when used as a root end filling material.

            M Torabinejad, T.F. Watson, T.R Pitt Ford (1993)
            This in vitro study used rhodamine B fluorescent dye and a confocal microscope to evaluate the sealing ability of amalgam, super EBA, and a mineral trioxide aggregate when used as root end filling materials. Thirty single-canal teeth were cleaned, shaped, and obturated with gutta-percha and root canal sealer. After application of nail polish to the external surface, the apical 3 mm of each root was resected and 3-mm deep root end preparations were made. The roots were randomly divided into three groups and the root end preparations filled with the experimental materials. All roots were then exposed to an aqueous solution of rhodamine B fluorescent dye for 24 h, longitudinally sectioned, and the extent of dye penetration measured using a confocal microscope. Statistical analysis showed that the mineral trioxide aggregate leaked significantly less than amalgam and super EBA.
            Article 0 comments Cited 221 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis.

              Hui Zhang, Ya Shen, N Dorin Ruse … (2009)
              The antibacterial effectiveness of 7 different endodontic sealers, AH Plus, Apexit Plus, iRoot SP, Tubli Seal, Sealapex, Epiphany SE, and EndoRez against Enterococcus faecalis was studied in vitro. A modified direct contact test was used. Bacteria in suspension were exposed to the materials for 2-60 minutes by using sealers that were freshly mixed or set for 1, 3, and 7 days. The pH values and contact angles of sterile water on sealers at different times after setting were also measured. Fresh iRoot SP killed all bacteria in 2 minutes, AH Plus in 5 minutes, EndoRez in 20 minutes, and Sealapex and Epiphany in 60 minutes. Freshly mixed Apexit Plus and Tubli Seal failed to kill all bacteria at 60 minutes. For 1-day and 3-day samples, iRoot SP and EndoRez had the strongest antibacterial activity, followed by Sealapex and Epiphany; Tubli Seal and AH Plus did n'ot show any significant antibacterial activity. Of all the samples, Apexit Plus had the lowest antimicrobial activity. The pH of the sealers could not alone explain their antibacterial effect. Fresh iRoot SP, AH Plus, and EndoRez killed E. faecalis effectively. IRoot SP and EndoRez continued to be effective for 3 and 7 days after mixing. Sealapex and EndoRez were the only ones with antimicrobial activity even at 7 days after mixing.
              Article 0 comments Cited 109 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (publisher-id): bor
                Title: Brazilian Oral Research
                Abbreviated Title: Braz. oral res.
                Publisher: Sociedade Brasileira de Pesquisa Odontológica - SBPqO (São Paulo, SP, Brazil )
                ISSN (Print): 1806-8324
                ISSN (Electronic): 1807-3107
                Publication date (Print and electronic): 2022
                Volume: 36
                Electronic Location Identifier: e063
                Affiliations
                [1] Araraquara São Paulo orgnameUniversidade Estadual Paulista orgdiv1Schoool of Dentistry orgdiv2Department of Restorative Dentistry Brazil
                [2] Araraquara São Paulo orgnameUniversidade Estadual Paulista orgdiv1Schoool of Dentistry orgdiv2Department of Diagnosis and Surgery Brazil
                Article
                Publisher ID: S1806-83242022000100256 Publisher ID: S1806-8324(22)03600000256
                DOI: 10.1590/1807-3107bor-2022.vol36.0063
                PubMed ID: 36507750
                SO-VID: 86dc11d3-1ec6-45cd-b4e0-0d07bf3feb9d
                License:

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                Date received : 08 February 2021
                Date accepted : 08 February 2022
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 41, Pages: 0
                Product

                SciELO Brazil

                Categories
                Subject: Original Research

                Keywords: Endodontics,Bone Morphogenetic Protein 2,Cell Proliferation,Cell Survival,Biocompatible Materials
                Data availability:
                Keywords: Endodontics, Bone Morphogenetic Protein 2, Cell Proliferation, Cell Survival, Biocompatible Materials

                Comments

                Comment on this article