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      Granulocytic ehrlichiosis in two dogs in Switzerland.

      Journal of Clinical Microbiology

      diagnosis, Animals, Thrombocytopenia, Switzerland, Sequence Homology, Nucleic Acid, Sequence Analysis, DNA, genetics, RNA, Ribosomal, 16S, Polymerase Chain Reaction, microbiology, Neutrophils, Male, Lymphoma, Non-Hodgkin, Leukopenia, Humans, Female, veterinary, blood, Ehrlichiosis, isolation & purification, Ehrlichia, Dogs, analysis, DNA, Bacterial

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          Abstract

          This case report describes two dogs with granulocytic ehrlichiosis. Dog 1 was a male Labrador retriever with clinical signs of lymphosarcoma. Dog 2 was a female Airedale terrier, whose clinical signs included apathy, pyrexia, diarrhea, and abdominal pain. Examination of blood smears revealed Ehrlichia organisms in the neutrophils of both dogs. There was thrombocytopenia in both dogs, and dog 2 also had leukopenia. In both dogs, bands of identical length were amplified from DNA of leukocytes via nested PCR. The bands had identical nucleotide sequences, which differed from the gene sequences of Ehrlichia equi and E. phagocytophila in three and two positions, respectively. Interestingly, the nucleotide sequence of the 16S rRNA was 100% homologous to that of a human granulocytic ehrlichia.

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          229959
          9276407

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