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Relationship between mRNA levels and protein accumulation in a chloroplast promoter-mutant of Chlamydomonas reinhardtii.

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      Abstract

      The photosynthetic chloroplast mutant G64 of Chlamydomonas reinhardtii was shown to contain a single point mutation within the 5' region of the psbD gene encoding the D2 protein of the photosystem II reaction center. The mutation affects the sequence element TATAATAT which has previously been hypothesized to function as the psbD promoter. Run-on analysis confirmed that transcription of psbD in the mutant was reduced to approximately 10% of the wild-type level. However, psbD mRNA accumulated to approximately 35%, despite the prominent decrease in RNA synthesis. This suggests that RNA-stabilization effects can compensate to some extent for a reduction in transcriptional activity. Interestingly, a direct correlation between transcript levels and the accumulation of the psbD gene product, the D2-protein, was observed in G64. The data suggest that posttranscriptionally acting regulatory factors determine the rate-limiting steps of chloroplast psbD gene expression.

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      Affiliations
      [1 ] Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Universitätsstr. 150, 44780 Bochum, Germany.
      Journal
      Mol. Genet. Genomics
      Molecular genetics and genomics : MGG
      Springer Nature America, Inc
      1617-4615
      1617-4623
      Dec 2005
      : 274
      : 6
      16244871
      10.1007/s00438-005-0056-x

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