9
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Glass capillary based microfluidic ELISA for rapid diagnostics.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics and fundamental biological research due to its high specificity and reproducibility. However, the traditional 96-well-plate based ELISA still suffers from several notable drawbacks, such as long assay time (4-6 hours), burdensome procedures and large sample/reagent volumes (∼100 μl), which significantly limit traditional ELISA's applications in rapid clinical diagnosis and quasi-real-time prognosis of some fast-developing diseases. Here, we developed a user friendly glass capillary array based microfluidic ELISA device. Benefiting from the high surface-to-volume ratio of the capillary and the rapid chemiluminescent photo-imaging method with a commercial camera, our capillary based ELISA device significantly reduced the sample volume to 20 μL and shortened the total assay time to as short as 16 minutes (including detection time), which represent approximately 10-fold and 5-fold reduction in assay time and sample volume, respectively, in comparison with the traditional plate-based method. Furthermore, through the double exposure method, a nearly 10-fold increase in the detection dynamic range was achieved over the traditional well-based ELISA. Our device can be broadly used in rapid biochemical analysis for biomedicine and research/development laboratories.

          Related collections

          Author and article information

          Journal
          Analyst
          The Analyst
          Royal Society of Chemistry (RSC)
          1364-5528
          0003-2654
          May 26 2017
          Affiliations
          [1 ] Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA. xsfan@umich.edu.
          [2 ] Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA. xsfan@umich.edu and Key Laboratory of Optical Fiber Sensing and Communications, University of Electronic Science and Technology of China, No. 2006, Xiyuan Ave., Chengdu, 611731, P. R. China.
          [3 ] Department of Chemistry, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA.
          Article
          10.1039/c7an00523g
          28548141
          8e96235b-457a-4896-9e7b-c7b6b5e5f23b
          History

          Comments

          Comment on this article