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      Effect of Dietary Salt and Cholesterol Loading on Vascular Adrenergic Receptors

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          Abstract

          To evaluate the effects of salt and cholesterol intake on vascular responses to catecholamines, α<sub>1</sub>- and β-adrenergic receptor densities were determined in control, cholesterol-loaded, salt-loaded with desoxycorticosterone acetate (DOCA) and furosemide-loaded male rabbits, using [<sup>3</sup>H]-prazosin and (–)-[<sup>125</sup>I]-cyanopindolol as ligands, respectively. In the aortic membrane, the density of α<sub>1</sub>-adrenergic receptors (B<sub>max</sub> = 120 ± 14 fmol/mg protein, K<sub>d</sub> = 0.48 ± 0.05 nM) was higher than that of β -adrenergic receptors (B<sub>max</sub> = 10.5 ± 1.7 fmol/mg protein, K<sub>d</sub> 47.1 ± 8.6 pM). Salt loading and depletion did not alter the density or affinity of either the α<sub>1</sub>- or β -adrenergic receptors. By contrast, cholesterol loading significantly decreased αi-adrenergic receptor affinity to a K<sub>d</sub> value of 0.81 ± 0.11 nMfrom the control level of 0.48 ± 0.05 and increased the β -adrenergic receptor density to a B<sub>max</sub> of 18.7 ± 1.9 fmol/mg protein from the control level of 10.5 ± 1.7. These results showed that the density of α<sub>1</sub>-adrenergic receptors was higher than that of β-adrenergic receptors in the rabbit aortic membrane preparation, and suggested that the sensitivity of aortic membrane to catecholamines was changed by cholesterol loading.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1988
          1988
          23 September 2008
          : 25
          : 5
          : 209-216
          Affiliations
          Department of Internal Medicine 2, Nagoya University School of Medicine, Nagoya, Japan
          Article
          158733 Blood Vessels 1988;25:209–216
          10.1159/000158733
          © 1988 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 8
          Categories
          Research Paper

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