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      Antibiotic resistance spectrum and genetic characteristics of MDRSA nasal isolate among primary school students

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          Abstract

          Objective To investigate the antibiotic resistance spectrum and genetic characteristics of multidrug-resistant Staphylococcus aureus (MDRSA) nasal isolate among primary school students, and to provide a scientific basis for the prevention and control of masal MDRSA resistance and the selection of clincal drugs in children.

          Methods Antibiotic susceptibility experiments were performed on all SA isolates of 1 705 primary school students from 8 primary schools in Guangzhou selected by using multistage cluster stratified sampling method. MDRSA antibiotic susceptibility spectrum was analyzed, and the resistant, virulence and immune evasion cluster ( IEC) genes detected by polymerase chain reaction (PCR) .

          Results The prevalence of MDRSA nasal carriage was 20.76%(354/1 705), and the proportion of multidrug resistance among SA isolates was 96.20% (354/368). The predominant resistant antibiotics of MDRSA isolates were penicillin (99.72%), erythromycin (96.33%), clindamycin (90.96%) and teicoplanin (90.11%). Notably, 240(67.80%, 240/354) MDRSA isolates were resistant to more than six antimicrobial categories. And the predominant detection rates of resistant genes were BlaZ (92.66%), Tet(M) (49.72%), virulence genes Tst (25.42%) and IEC genes Sak (92.09%), Hlb (61.58%).

          Conclusion We found high prevalence of nasal colonization MDRSA from healthy children. Moreover, MDRSA isolates has a high resistant rate to multiple antibiotics, and the proportion of resistant to ≥ 6 antimicrobial categories is high.

          Abstract

          【摘要】 目的 探究小学生鼻腔多重耐药金黄色葡萄球菌(multidmg-resistant staphylococcus aureus, MDRSA)的耐药 谱和基因携带特征,为预防和控制儿童鼻腔MDRSA耐药产生及临床用药的选择提供科学依据。 方法采用多阶段分层 整群抽样方法,抽取广州市8所小学1 705名小学生,对儿童鼻腔分离鉴定出的金黄色葡萄球菌(SA)进行药物敏感实验, 分析MDRSA的耐药谱,用多重聚合酶链式反应(PCR)技术测定耐药、毒素和免疫逃避(immune evasion cluster, IEC)基因。 结果 MDRSA 携带率为 20.76%(354/1 705),MDRSA 占 SA 的比例为 96.20%(354/368)。MDRSA 对青霉素(99.72%)、红 霉素(96.33%)、克林霉素(90.96%)和替考拉宁(90.11%)的耐药率髙,同时对6类及以上抗生素耐药率为67.80%(240/ 354)。主要携带耐药基因 BlaZ (92.66%)、 Tet ( M) (49.72%),毒素基因 Tst (25.42%),IEC 基因 Sak (92.09%)和 Hlb (61.58%)。结论健康儿童鼻腔MDRSA携带率较髙,MDRSA对多种抗生素有髙耐药率,且耐药类别≥6的比例髙。

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          Author and article information

          Journal
          CJSH
          Chinese Journal of School Health
          Chinese Journal of School Health (China )
          1000-9817
          01 June 2020
          01 June 2020
          : 41
          : 6
          : 908-910
          Affiliations
          [1] 1School of Public Health, Guangdong Pharmaceutical University, Guangzhou (510310), China
          Author notes
          *Corresponding author: YAO Zhenjiang, E-mail: zhjyao2001@ 123456yahoo.com
          Article
          j.cnki.1000-9817.2020.06.029
          10.16835/j.cnki.1000-9817.2020.06.029
          8f5f85e2-97df-49a4-a469-f0993d94cb39
          © 2020 Chinese Journal of School Health

          This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 Unported License (CC BY-NC 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc/4.0/.

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          Self URI (journal-page): http://www.cjsh.org.cn
          Categories
          Journal Article

          Ophthalmology & Optometry,Pediatrics,Nutrition & Dietetics,Clinical Psychology & Psychiatry,Public health
          Staphylococcus aureus,Genes,Microbial sensitivity tests,Students,Nasal cavity

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