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Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR
December 1999
December 1999
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Abstract
A primer pair (p289/290) based on the RNA polymerase sequence of 25 prototype and
currently circulating strains of human caliciviruses (HuCVs) was designed for the
detection of both Norwalk-like caliciviruses (NLVs) and Sapporo-like caliciviruses
(SLVs) by reverse transcription-polymerase chain reaction (RT-PCR). This primer pair
produces RT-PCR products of 319 bp for NLVs and 331 bp for SLVs. The usefulness of
this primer pair was shown by its detection of prototype NLVs (Norwalk, Snow Mountain,
Hawaii and Mexico viruses) and SLVs (Sapporo/82, Hou/86, Hou/90 and Lon/92) and currently
circulating strains of NLVs and SLVs in children and adults. This primer pair also
detected more viruses in either NLV or SLV genera than previously designed primers.
This primer pair is useful for broad detection of HuCVs for clinical and epidemiologic
studies as well as for environmental monitoring.