To biologically inhibit the production of highly toxic sulfide in the aquaculture area, one bacterium, designated as Hydrogenovibrio thermophilus strain TT, was isolated from sediment in Jiaozhou Bay, China. The strain was found to be microaerobic mixtrophic, employing both carbon dioxide and organic carbons, e.g. citrate, lactate, glucose, and peptone as carbon sources. When it oxidized the sulfide and thiosulfate, oxygen was the optimal electron acceptor, followed by nitrite and nitrate, which benefited to removal of sulfide under low-oxygen mariculture environment. In addition, the strain TT exhibited remarkable tolerance of sulfide and thiosulfate in seawater, and it was able to oxidize 18.52 mmol L −1 S 2− in 12 h or 45 mmol L −1 S 2O 3 2− in 24 h at initial pH 7.0–9.0, 30–40°C. Therefore, this strain showed a quite promising application for biological remediation of sulfide-contaminated mariculture system.