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      Munc18-1 promotes large dense-core vesicle docking.

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      Publication date:
      Journal: Neuron
      Keywords: Animals, Antigens, Surface, metabolism, Cattle, Cell Membrane, Chromaffin Cells, ultrastructure, Exocytosis, physiology, Female, Fetus, cytology, Gene Deletion, Gene Expression, Membrane Potentials, Mice, Mice, Mutant Strains, Microscopy, Electron, Munc18 Proteins, Nerve Tissue Proteins, genetics, Patch-Clamp Techniques, Pregnancy, Synaptic Vesicles, Syntaxin 1, Vesicular Transport Proteins

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          Abstract

          Secretory vesicles dock at the plasma membrane before Ca(2+) triggers their exocytosis. Exocytosis requires the assembly of SNARE complexes formed by the vesicle protein Synaptobrevin and the membrane proteins Syntaxin-1 and SNAP-25. We analyzed the role of Munc18-1, a cytosolic binding partner of Syntaxin-1, in large dense-core vesicle (LDCV) secretion. Calcium-dependent LDCV exocytosis was reduced 10-fold in mouse chromaffin cells lacking Munc18-1, but the kinetic properties of the remaining release, including single fusion events, were not different from controls. Concomitantly, mutant cells displayed a 10-fold reduction in morphologically docked LDCVs. Moreover, acute overexpression of Munc18-1 in bovine chromaffin cells increased the amount of releasable vesicles and accelerated vesicle supply. We conclude that Munc18-1 functions upstream of SNARE complex formation and promotes LDCV docking.

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          PubMed ID:: 11545717
          DOI:: 10.1016/S0896-6273(01)00391-9

          ScienceOpen disciplines: Chemistry
          Keywords: Animals,Antigens, Surface,metabolism,Cattle,Cell Membrane,Chromaffin Cells,ultrastructure,Exocytosis,physiology,Female,Fetus,cytology,Gene Deletion,Gene Expression,Membrane Potentials,Mice,Mice, Mutant Strains,Microscopy, Electron,Munc18 Proteins,Nerve Tissue Proteins,genetics,Patch-Clamp Techniques,Pregnancy,Synaptic Vesicles,Syntaxin 1,Vesicular Transport Proteins
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          ScienceOpen disciplines: Chemistry
          Keywords: Animals, Antigens, Surface, metabolism, Cattle, Cell Membrane, Chromaffin Cells, ultrastructure, Exocytosis, physiology, Female, Fetus, cytology, Gene Deletion, Gene Expression, Membrane Potentials, Mice, Mice, Mutant Strains, Microscopy, Electron, Munc18 Proteins, Nerve Tissue Proteins, genetics, Patch-Clamp Techniques, Pregnancy, Synaptic Vesicles, Syntaxin 1, Vesicular Transport Proteins

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