Borrelia burgdorferi s. lato have been recovered by means of an observations in a dark screen, phase and anoptrail contrasts in 87.47% imago and 100% nymphs and larvae of the Ixodes persulcatus ticks collected in the Leningrad, Novgorod and Pskov regions. Four morphological types of borreliae have been recognized, which were present in all stages of ticks examined. The location of the agent in ticks was examined in the material coloured after Levaditi. In the hungry stages and the most abundance of the agent was accumulated at the apical surface of midgut epithelium. In the majority of hungry I. persulcatus the generalized infection was recorded. The feeding stimulates the migration of the agent through the intracellular space to the basal membrane and cells of organs and finally leads to complication of the agent in the organism of vector.