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      Neovascularization of Embryonic Rat Hearts Cultured in oculo Closely Mimics in utero Coronary Vessel Development

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          Abstract

          Coronary neovascularization was studied following grafting of avascular hearts from gestation day-12 (E-12) rat embryos to the anterior eye chambers of adult rats. Volume densities (V<sub>v</sub>) of vessels, myocytes, and the extracellular matrix (ECM) after 3–7, 14, 21, and 35 days in oculo were compared to V<sub>v</sub> in hearts developing in utero at E-15, E-18, and E-20. The myocardium in both models exhibited similar vessel V<sub>v</sub> and capillary developmental stages: (1) clustering of endothelial cells and red blood cells; (2) endothelial cell migration, and (3) tube formation/maturation. The V<sub>v</sub> of myocytes increased while that of the ECM remained constant over time. Cross-species grafting utilizing species-specific antibodies determined that the majority, but not all, of the 10-day graft vasculature was of graft origin. Therefore, both de novo growth (vasculogenesis) and sprouting (angiogenesis) were occurring in oculo. Tracer molecules infused into host rats reached the outermost graft vessels only after 10 days in oculo, suggesting a functional link with the host circulation after this time. Thus, we have shown that both models exhibit similar: (1) vascular V<sub>v</sub>; (2) shifts in V<sub>v</sub> of nonvascular components; (3) stages of neovascularization, and (4) mechanisms of neovascularization. In conclusion, coronary neovascularization occurring in oculo closely mimics normal coronary vessel development.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1994
          1994
          23 September 2008
          : 31
          : 4
          : 205-215
          Affiliations
          aDepartment of Anatomy and Cardiovascular Center, University of Iowa, Iowa City, Iowa, and bDepartment of Psychology and Physiology, Neurobiology Research Center, UAB Station, University of Alabama at Birmingham, Ala., USA
          Article
          159045 J Vasc Res 1994;31:205–215
          10.1159/000159045
          7517702
          © 1994 S. Karger AG, Basel

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          Page count
          Pages: 11
          Categories
          Research Paper

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