With a combination of non-isotopic in situ hybridization and simultaneous fluorescent R-banding, this study presents the first map of the short arm of sheep Chromosome (Chr) 3 and of the homoeologous cattle and goat Chrs 11 with two DNA sequences: the beta lactoglobulin gene (LGB) and an immunoglobulin M heavy chain-like sequence (IGHML). The results are in agreement with the high degree of banding pattern similarity, previously reported, among cattle, sheep, and goat karyotypes.