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A simple method using a polymethylpenten chamber for isolation of human pancreatic islets.
Denis Dufrane
1 ,
Rose-Marie Goebbels ,
Yves Guiot ,
Jean-Paul Squifflet ,
Jean-Claude Henquin ,
Pierre Gianello
Apr 2005
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Abstract
Isolation of large numbers of intact and functional human islets remains difficult and expensive. We describe a novel method using a polymethylpenten chamber (PMPC) and compare its efficacy to the classic method using a stainless steel chamber (SSC).