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      Spatial transcriptomic analysis of the mouse brain following chronic social defeat stress

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          Abstract

          Depression is a highly prevalent and disabling mental disorder, involving numerous genetic changes that are associated with abnormal functions in multiple regions of the brain. However, there is little transcriptomic‐wide characterization of chronic social defeat stress (CSDS) to comprehensively compare the transcriptional changes in multiple brain regions. Spatial transcriptomics (ST) was used to reveal the spatial difference of gene expression in the control, resilient (RES) and susceptible (SUS) mouse brains, and annotated eight anatomical brain regions and six cell types. The gene expression profiles uncovered that CSDS leads to gene synchrony changes in different brain regions. Then it was identified that inhibitory neurons and synaptic functions in multiple regions were primarily affected by CSDS. The brain regions Hippocampus (HIP), Isocortex, and Amygdala (AMY) present more pronounced transcriptional changes in genes associated with depressive psychiatric disorders than other regions. Signalling communication between these three brain regions may play a critical role in susceptibility to CSDS. Taken together, this study provides important new insights into CSDS susceptibility at the ST level, which offers a new approach for understanding and treating depression.

          Abstract

          In this study, Wang et al. explored the potential pathogenesis of chronic social defeat stress (CSDS)‐induced depressive‐like behaviour in mice using 10× Visium spatial transcriptomics (ST) technique. Many of the depression‐related gene changes were identified and more pronounced in the hippocampus (HIP), cortex (Isocortex), and amygdala (AMY) in susceptible (SUS) mice compared to control and resilient (RES) mice. In particular, compared to other cell types, inhibitory interneurons, and synaptic function were most significantly affected in multiple brain regions following CSDS.

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          Author and article information

          Contributors
          wuht@bmi.ac.cn
          Journal
          Exploration (Beijing)
          Exploration (Beijing)
          10.1002/(ISSN)2766-2098
          EXP2
          Exploration
          John Wiley and Sons Inc. (Hoboken )
          2766-8509
          2766-2098
          13 October 2023
          December 2023
          : 3
          : 6 ( doiID: 10.1002/exp2.v3.6 )
          : 20220133
          Affiliations
          [ 1 ] Department of Neurobiology Beijing Institute of Basic Medical Sciences Beijing China
          [ 2 ] Neuroscience Research Center, School of Medicine Shahid Beheshti University of Medical Sciences Tehran Iran
          [ 3 ] Key Laboratory of Neuroregeneration, Co‐innovation Center of Neuroregeneration Nantong University Nantong China
          [ 4 ] Chinese Institute for Brain Research Beijing China
          Author notes
          [*] [* ] Correspondence

          Haitao Wu, Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 100850 Beijing, China.

          Email: wuht@ 123456bmi.ac.cn

          Author information
          https://orcid.org/0000-0001-6526-5147
          https://orcid.org/0000-0001-8437-3194
          Article
          EXP20220133
          10.1002/EXP.20220133
          10742195
          38264685
          9ad90089-7a64-409c-b8a3-3f6901a882b0
          © 2023 The Authors. Exploration published by Henan University and John Wiley & Sons Australia, Ltd.

          This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

          History
          : 03 June 2023
          : 03 September 2023
          Page count
          Figures: 7, Tables: 1, Pages: 15, Words: 9491
          Funding
          Funded by: National Key Research and Development Program of China , doi 10.13039/501100012166;
          Award ID: 2021YFA1101801
          Award ID: 2021ZD0202500
          Funded by: National Natural Science Foundation of China , doi 10.13039/501100001809;
          Award ID: 32325025
          Award ID: 31770929
          Award ID: 32171148
          Categories
          Research Article
          Research Articles
          Custom metadata
          2.0
          December 2023
          Converter:WILEY_ML3GV2_TO_JATSPMC version:6.3.6 mode:remove_FC converted:22.12.2023

          chronic social defeat stress,depression,gene expression,spatial transcriptomics

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