3
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Next-Generation Sequencing RNA-Seq Library Construction.

      Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
      library construction, transcriptome, RNA-Seq, NGS, gene expression, strand-specific

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA library preparation set for Illumina, although similar kits exist from different vendors. The protocol preserves the orientation of the original RNA in the final sequencing library, enabling strand-specific analysis of the resulting data. These libraries have been used for differential gene expression analysis and small RNA discovery and are currently being tested for de novo transcriptome assembly. The protocol is robust and applicable to a broad range of RNA input types and RNA quality, making it ideal for high-throughput laboratories.

          Related collections

          Author and article information

          Journal
          24733242
          10.1002/0471142727.mb0421s106

          library construction,transcriptome,RNA-Seq,NGS,gene expression,strand-specific

          Comments

          Comment on this article

          scite_