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      mRNA Expression of the IGF System in the Kidney of the Hypersomatotropic Rat


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          Aim: To examine the response of the insulinlike growth factor (IGF) system in the kidney during a state of extreme growth. Methods: We studied the mRNA expression of IGF-I, IGF-I receptor, and IGF-binding proteins (BP) using sensitive RNase protection assays following subcutaneous implantation of growth hormone pituitary cells (GH<sub>3</sub>) in rats. Results: Within 5 weeks, the serum GH levels increased from 18.0 ± (SE) 5.0 ng/ml in control animals to 389.8 ± 30.3 ng/ml in GH<sub>3</sub> rats (n = 5, p < 0.001). The circulating IGF-I levels were also elevated. The kidney weights increased from 0.74 ± 0.01 g in controls to 1.06 ± 0.03 g in GH<sub>3</sub> animals (n = 5, p < 0.001). Similar changes were observed at week 10. The renal IGF-I mRNA averaged 1.0 ± (SD) 0.33 relative densitometry units in controls (n = 4) and increased to 2.11 ± 0.13 relative densitometry units in GH<sub>3</sub> rats (n = 5, p < 0.001). On the other hand, mRNA for the type I IGF receptor decreased in hypersomatotropic rats. Messenger RNAs for IGFBP-1 and IGFBP-4, which have been localized to renal tubules, both decreased significantly following growth induction, while IGFBP-3, the mRNA of which has an interstitial localization, was increased at week 10. Conclusion: These data suggest that there is a dynamic relationship between tubular and interstitial compartments with regard to the IGF system in the kidney which may be important in the regulation of the cell mass.

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          Author and article information

          S. Karger AG
          25 July 2001
          : 88
          : 4
          : 360-367
          aDivision of Nephrology, Department of Medicine, University of Maryland Medical School, Baltimore, Md., and bSurgical Research Laboratory, Children’s Hospital, Boston, Mass., USA; cDepartments of Pediatrics and Neurology and Neurosurgery, McGill University and Montreal Children’s Hospital, Montreal, Que., Canada
          46021 Nephron 2001;88:360–367
          © 2001 S. Karger AG, Basel

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          Page count
          Figures: 4, Tables: 2, References: 33, Pages: 8
          Self URI (application/pdf): https://www.karger.com/Article/Pdf/46021
          Self URI (text/html): https://www.karger.com/Article/FullText/46021
          Self URI (journal page): https://www.karger.com/SubjectArea/Nephrology
          Original Paper

          Cardiovascular Medicine,Nephrology
          Kidney, growth,Carrier proteins,IGF receptors
          Cardiovascular Medicine, Nephrology
          Kidney, growth, Carrier proteins, IGF receptors


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