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      Two new Brazilian species of Loxosceles Heinecken & Lowe, 1832 with remarks on amazonica and rufescens groups (Araneae, Sicariidae)

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          Abstract

          Abstract

          The genus Loxosceles Heinecken & Lowe, 1832 has 91 representatives in the New World. Despite medical relevancy, the taxonomy of the genus is poorly understood. South American Loxosceles were divided into four groups of species: laeta, spadicea, gaucho and amazonica ; this last one has a single species, Loxosceles amazonica Gertsch, 1967. More recently, the natural occurrence of L. amazonica in the New World has been questioned, due to the strong morphological resemblance and close phylogenetic relationship with Old World species, mainly with Loxosceles rufescens (Dufour, 1820). Herein, L. amazonica is rediagnosed and its morphological variation and natural distribution discussed. Two new species closely related to it from northeastern Brazil are also described, Loxosceles willianilsoni sp. n., from the state of Rio Grande do Norte, and Loxosceles muriciensis sp. n., from the state of Alagoas . The relationships of these new species with L. amazonica and L. rufescens are discussed.

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          Phylogenetic relationships of Loxosceles and Sicarius spiders are consistent with Western Gondwanan vicariance.

          The modern geographic distribution of the spider family Sicariidae is consistent with an evolutionary origin on Western Gondwana. Both sicariid genera, Loxosceles and Sicarius are diverse in Africa and South/Central America. Loxosceles are also diverse in North America and the West Indies, and have species described from Mediterranean Europe and China. We tested vicariance hypotheses using molecular phylogenetics and molecular dating analyses of 28S, COI, 16S, and NADHI sequences. We recover reciprocal monophyly of African and South American Sicarius, paraphyletic Southern African Loxosceles and monophyletic New World Loxosceles within which an Old World species group that includes L. rufescens is derived. These patterns are consistent with a sicariid common ancestor on Western Gondwana. North American Loxosceles are monophyletic, sister to Caribbean taxa, and resolved in a larger clade with South American Loxosceles. With fossil data this pattern is consistent with colonization of North America via a land bridge predating the modern Isthmus of Panama.
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            The spider genus Loxosceles in South America (Araneae, Scytodidae).

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              Diversity of Loxosceles spiders in Northwestern Africa and molecular support for cryptic species in the Loxosceles rufescens lineage.

              Until recently, Loxosceles rufescens was the only species known from a geographic range including Northern Africa, Mediterranean Europe and the Middle East. Rich Loxosceles diversity in the New World suggests either that L. rufescens is a young lineage or that its diversity is underappreciated. We use a molecular phylogenetic and morphological approach to examine diversity in L. rufescens and other Loxosceles lineages in Northwestern Africa. Molecular analyses of one nuclear and two mitochondrial genes strongly support a monophyletic clade including L. rufescens, the Northern Brazilian L. amazonica and three other divergent Northwestern African lineages, though relationships among them remain unresolved. A genetically divergent Moroccan individual morphologically consistent with L. rufescens was strongly supported as sister to all other putative L. rufescens, consistent with the presence of at least 2 species in this lineage. COI p-distances and population structuring among remaining putative L. rufescens clades further suggest the absence of gene flow between clades and the possibility that they represent multiple species. Morphological characters of preserved Loxosceles collected in a range of African countries provide additional indication that Loxosceles are more diverse and have a deeper history in Africa than has been previously understood. (c) 2009 Elsevier Inc. All rights reserved.
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                Author and article information

                Journal
                Zookeys
                Zookeys
                ZooKeys
                ZooKeys
                Pensoft Publishers
                1313-2989
                1313-2970
                2017
                10 April 2017
                : 667
                : 67-94
                Affiliations
                [1 ] Laboratório Especial de Ecologia e Evolução, Instituto Butantan, Av. Vital Brazil, 1500 CEP 05503-900, São Paulo, São Paulo, Brazil
                [2 ] Fundação Museu do Homem Americano, Laboratório de Vestígios Orgânicos, Centro Cultural Sérgio Motta s/n, CEP 64770-000, Caixa Postal 2, São Raimundo Nonato, Piauí, Brazil
                Author notes
                Corresponding author: Caroline Sayuri Fukushima ( carolinesayuri@ 123456gmail.com )

                Academic editor: Cor Vink

                Article
                10.3897/zookeys.667.11369
                5523387
                a1da52aa-7d31-4e75-a0ed-ed4b3c6b2bd4
                Caroline Sayuri Fukushima, Rute Maria Gonçalves de Andrade, Rogério Bertani

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 29 November 2016
                : 12 March 2017
                Funding
                Funded by: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 501100002322 http://doi.org/10.13039/501100002322
                Categories
                Research Article

                Animal science & Zoology
                alagoas,brown recluse spider,caatinga,cave,rio grande do norte,animalia,araneae,sicariidae

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