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      Absence of Cytokine Response to Bacterial Challenge in Human Tubuloepithelial Cells

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          In acute bacterial renal infections, which are most frequently caused by Escherichia coli, tubuloepithelial cells are involved with respect to bacterial adherence, invasion and cytotoxicity. In addition, cytokines expressed by tubuloepithelial cells may be relevant for the recruitment of inflammatory cells and tissue damage in bacterial interstitial nephritis. We asked which inflammatory cytokines are produced by primary human tubuloepithelial cells following in vitro exposure to E. coli and found no release of IL-6, IL-8 and TNF-α by tubular cells challenged by bacteria. Purified virulence factors (fimbriae, lipopolysaccharide) from E. coli were also without effects on cytokine release by tubular cells. Since lymphocytic infiltration is a characteristic feature in the chronic form of interstitial nephritis, MHC class II expression by tubular cells in response to bacterial coincubation was analyzed. Exposure to both IFN-γ and E. coli enhanced MHC class II expression on tubuloepithelial cells. In conclusion, tubuloepithelial cells may play an active role in the local defense against bacteria, e.g. by expressing MHC class II molecules. However, in vitro inflammatory cytokines are not induced by E. coli in this cell population.

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          Costimulatory B7 molecules in the pathogenesis of infectious and autoimmune diseases.

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            • Article: not found

            Transfection Alters Ion Transport in MDCK Cells


              Author and article information

              Kidney Blood Press Res
              Kidney and Blood Pressure Research
              S. Karger AG
              29 June 2001
              : 24
              : 2
              : 105-110
              aMedizinische Klinik I, Medizinische Universität zu Lübeck und bForschungsinstitut Borstel, Deutschland
              54215 Kidney Blood Press Res 2001;24:105–110
              © 2001 S. Karger AG, Basel

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              Figures: 3, Tables: 1, References: 42, Pages: 6
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