Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathy

Abstract

Objectives:

To examine the impact of serum-derived bovine immunoglobulin, an oral medical food known to neutralize bacterial antigen and reduce intestinal inflammation, on restoration of mucosal immunity and gastrointestinal function in individuals with HIV enteropathy.

Design:

Open-label trial with intensive 8-week phase of bovine serum immunoglobulin (SBI) 2.5 g twice daily with a 4-week washout period and an optional 9-month extension study.

Methods:

HIV enteropathy was defined as chronic gastrointestinal symptoms including frequent loose or watery stools despite no identifiable, reversible cause. Upper endoscopy for tissue immunofluorescent antibody assay and disaccharide gut permeability/absorption studies were performed before and after 8 weeks of SBI to test mucosal immunity and gastrointestinal function. Blood was collected for markers of microbial translocation, inflammation, and collagen kinetics. A validated gastrointestinal questionnaire assessed changes in symptoms.

Results:

All eight participants experienced profound improvement in symptoms with reduced bowel movements/day ( P = 0.008) and improvements in stool consistency ( P = 0.008). Gut permeability was normal before and after the intervention, but d-xylose absorption increased in seven of eight participants. Mucosal CD4 ⁺ lymphocyte densities increased by a median of 139.5 cells/mm ² from 213 to 322 cells/mm ² ( P = 0.016). Intestinal-fatty acid binding protein (I-FABP), a marker of enterocyte damage, initially rose in seven of eight participants after 8 weeks ( P = 0.039), and then fell below baseline in four of five who continued receiving SBI ( P = 0.12). Baseline serum I-FABP levels were negatively correlated with subsequent rise in mucosal CD4 ⁺ lymphocyte densities ( r = −0.74, P = 0.046).

Conclusion:

SBI significantly increases intestinal mucosal CD4 ⁺ lymphocyte counts, improves duodenal function, and showed evidence of promoting intestinal repair in the setting of HIV enteropathy.