Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB.

Applied and Environmental Microbiology

Software, Archaea, classification, genetics, Bacteria, Databases, Nucleic Acid, standards, Genes, rRNA, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Recombination, Genetic, Reproducibility of Results, Sequence Alignment

Read this article at

ScienceOpenPublisherPMC
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      A 16S rRNA gene database (http://greengenes.lbl.gov) addresses limitations of public repositories by providing chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies. It was found that there is incongruent taxonomic nomenclature among curators even at the phylum level. Putative chimeras were identified in 3% of environmental sequences and in 0.2% of records derived from isolates. Environmental sequences were classified into 100 phylum-level lineages in the Archaea and Bacteria.

      Related collections

      Author and article information

      Journal
      10.1128/AEM.03006-05
      1489311
      16820507

      Comments

      Comment on this article