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      Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics

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          Jiawei Shengjiang Powder (JWSJP) is a classical Chinese medicinal formula, which has been widely applied in the treatment of asthma and complications for many years due to its curative effect.


          To verify the effect of JWSJP in improving abnormal sperm motility caused by asthma and to explore its potential mechanism.

          Materials and Methods

          The active compounds of JWSJP were obtained from high performance liquid chromatography tandem mass spectrometry and the Traditional Chinese Medicine System Pharmacology. The key active components and targets of JWSJP were predicted based on network pharmacological analysis and bioinformatics research. Rats were randomly divided into normal, model and treatment groups. The rat model of allergic asthma was induced by intraperitoneal injection of ovalbumin solution. The experiment judged improvement of semen quality by evaluating sperm motility, and detected the expression of related proteins in testicular tissue of Sprague-Dawley rats by RT-qPCR and Western blot methods. Hematoxylin and eosin (HE) staining was used to observe the changes in testicular tissue structure in rats.


          Through the analysis of network pharmacology and bioinformatics, it was found that beta-sitosterol, quercetin, gallic acid, pelargonidin and kaempferol were the key active components of Jiawei Shengjiang Powder. Tumor necrosis factor (TNF), interleukin-6 (IL-6) and insulin (INS) genes are crucial targets of JWSJP in the treatment of spermatogenic dysfunction caused by acute asthma. After 8 weeks of intervention, compared with the model group, the treatment group had significantly improved sperm motility (P < 0.05). There were significant differences in TNF, IL6, and INS proteins in the treatment group, and the HE staining of testicular tissue structure in the treatment group was significantly improved.


          JWSJP can improve the abnormal sperm motility induced by asthma, and its mechanism may be related to the expression of related proteins and mRNA of TNF, IL6, and INS.

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          Most cited references 43

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                Author and article information

                Drug Des Devel Ther
                Drug Des Devel Ther
                Drug Design, Development and Therapy
                19 March 2021
                : 15
                : 1245-1259
                [1 ]First Clinical Medical College, Beijing University of Chinese Medicine , Beijing, 100029, People’s Republic of China
                [2 ]Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine , Beijing, 100700, People’s Republic of China
                [3 ]The Third Affiliated Hospital of Beijing University of Chinese Medicine , Beijing, 100029, People’s Republic of China
                [4 ]Pneumology Department, The Third Affiliated Hospital of Beijing University of Chinese Medicine , Beijing, 100029, People’s Republic of China
                [5 ]Department of Andrology, The First Affiliated Hospital of Henan University of Chinese Medicine , Zhengzhou, 450046, People’s Republic of China
                Author notes
                Correspondence: Bin Wang Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine , Beijing, 100700, People’s Republic of China Email dayiwangbln@sina.com
                Hong-sheng Cui Pneumology Department, The Third Affiliated Hospital of Beijing University of Chinese Medicine , Beijing, 100029, People’s Republic of China Email Hshcui@sina.com

                These authors contributed equally to this work

                © 2021 Wang et al.

                This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License ( http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms ( https://www.dovepress.com/terms.php).

                Page count
                Figures: 7, Tables: 5, References: 43, Pages: 15
                Funded by: the National Natural Science Foundation of China;
                Funded by: university-level project of Beijing University of Chinese Medicine;
                This study was supported by the National Natural Science Foundation of China (grant Number: 81774320) and university-level project of Beijing University of Chinese Medicine (NO.2019-XS-ZB04).
                Original Research


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